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John Cullen, VMD, PhD

Board Certification:

Diplomate: American College of Veterinary Pathology

Professional Experience

1994-present Professor of Veterinary Pathology, College of Veterinary Medicine, North Carolina State University

1989-1994 Associate Professor of Veterinary Pathology, College of Veterinary Medicine, North Carolina State University

1988-present Toxicology Faculty, North Carolina State University

1984-1989 Assistant Professor of Veterinary Pathology, College of Veterinary Medicine, North Carolina State University

1983-1984 Senior Resident in Anatomic Pathology, Veterinary Medical Teaching Hospital, University of California at Davis

1979-1983 Resident, Anatomic Pathology, School of Veterinary Medicine, University of California at Davis

1976-1979 Private practice, small animal clinician

1975-1976 Intern, Angell Memorial Animal Hospital, Boston, Massachusetts

Research Area

• Drug-induced hepatic disease
• Animal Models of viral hepatitis

Current Research

• Hepatic toxicity
  Comparisons of acute hepatotoxicity in conventional and germ free mice
  Acute biliary toxicity in IL-10 k.o. mice
  Acute biliary toxicity in Kupffer cell depleted mice
  Acute gene expression in biliary epithelia following oral ANIT exposure
• Animal models of viral hepatitis
  Augmented virtual reality guidance for RFA of woodchuck hepatocellular carcinoma

Current Collaborators

• The Hamner Institute
• Safety Assessment, GlaxoSmithKline
• Dr. Henry Fuchs, UNC, Dept. of Computer Science.

Publications

2010 Faiola B, Peterson R, Jordan, H, Kimbrough CA, Cullen JM. Acute ANIT Toxicity in Male IL-10 Knockout and Wild Type Mice. Tox Pathol (In press)

2010Cullen J M, Falls J G, Brown HR, Yoon LW, Cariello N F, Faiola B, Kimbrough C L, Jordan HL, Miller R T. Time Course Gene Expression using Laser Capture Microscopy-extracted Bile Ducts, But Not Hepatic Parenchyma, Reveals Acute Alpha-Napthylisothiocyante Toxicity. Tox Pathol (In press)

2010 Zadrozny LM, Williams CV, Remick AK, Cullen JM Spontaneous Hepatocellular Carcinoma in Captive Prosimians..Vet Pathol. 2010 Feb

2010 Brown DL, Van Winkle T, Cecere T, Rushton S, Brachelente C, Cullen JM. Congenital hepatic fibrosis in 5 dogs. Vet Pathol. 47:102-7.

2009 Cullen JM. Summary of the world small animal veterinary association standardization committee guide to classification of liver disease in dogs and cats. Vet Clin North Am Small Anim Pract. 39:395-418

2009 Cullen JM, Brown DL, Kissling GE, Foley JF, Rizzo J, Marion PL, Parron VI, French JE.Aflatoxin B1 and/or hepatitis B virus induced tumor spectrum in a genetically engineered hepatitis B virus expression and Trp53 haploinsufficient mouse model system for hepatocarcinogenesis.Toxicol Pathol.37:333-42.

2008 Cullen JM, Lindsey-Pegram D, Cote PJ. Serologic survey of woodchuck hepatitis virus in North Carolina woodchucks (Marmota monax).J Zoo Wildl Med. 39:263-5

2008 Ranck RS, Cullen JM, Waggie KS, Marion PL  Harderian gland neoplasms in captive, wild-caught Beechey ground squirrels (Spermophilus beecheyi). Vet Pathol. 45:388-92

2008 Macias E, de Marval PL, Senderowicz A, Cullen J, Rodriguez-Puebla ML.Expression of CDK4 or CDK2 in mouse oral cavity is retained in adult pituitary with distinct effects on tumorigenesis. Cancer Res. 68:162-71

2007 Brown DL, Anderson M, Cullen JM,  Mesenchymal hamartoma of the liver in a late-term equine fetus..Vet Pathol. 44:100-2.

2007 Hess PR, Barnes C, Woolard MD, Johnson MD, Cullen JM, Collins EJ, Frelinger JA.Selective deletion of antigen-specific CD8+ T cells by MHC class I tetramers coupled to the type I ribosome-inactivating protein saporin. Blood.  15:3300-7

2006 Johnson AL, Divers TJ, Freckleton ML, McKenzie HC, Mitchell E, Cullen JM, McDonough SP.Fall panicum (Panicum dichotomiflorum) hepatotoxicosis in horses and sheep. J Vet Intern Med. 20:1414-21

2006 Cullen J M    Miller R T, The role of pathology in the identification of drug-induced hepatic toxicity. Expert Opinion on Drug Metabolism & Toxicology , Vol. 2, No. 2, Pgs 241-247
2005 Cullen JM. Mechanistic classification of liver injury. Toxicol Pathol. 33: 6-8.

2005 Hajjou M, Norel R, Carver R, Marion P, Cullen J, Rogler LE, Rogler CE. cDNA microarray analysis of HBV transgenic mouse liver identifies genes in lipid biosynthetic and growth control pathways affected by HBV. J, Med Virol. 77: 57-65.

2005 Elmore SA, Basseches J, Anhalt GJ, Cullen JM, Olivry T. Paraneoplastic pemphigus in a dog with splenic sarcoma. Vet Pathol. 42: 88-91.

2004 Zhu Y, Cullen JM, Aldrich CE, Saputelli J, Miller D, Seeger C, Mason WS, Jilbert AR. Adenovirus-based gene therapy during clevudine treatment of woodchucks chronically infected with woodchuck hepatitis virus. Virol. 327:26-40.

2004 Li DH, Kumanogoh A, Cao TM, Parnes JR, Cullen JM. Woodchuck interleukine-6 gene: structure, characterization, and biologic activity. Gene. 342: 157-164.

2001 Cullen JM, Li DH, Brown C, Eisenberg GC, Wolfe KC, Toole J, Gibbs C Antiviral Efficacy and Pharmacokinetics of Oral Adefovir Dipivoxil in Chronically WHV-infected Woodchucks. Antimicrob Agents Chemother. 45:2740-5

2001 Zhu, Y., Yamamoto, T., Cullen, J., Saputelli, J., Aldrich C.E., Miller, D.S., Litwin, S., Furman, P.A., Jilbert, A.R., Mason, W.S. Kinetics of hepadnavirus loss from the liver during inhibition of viral DNA synthesis. J Virol, 75:311-322.

2001 Hervas-Stubbs S, Lasarte JJ, Sarobe P, Vivas I, Condreay L, Cullen JM, Prieto J,Borras-Cuesta F. T-helper cell response to woodchuck hepatitis virus antigens after therapeutic vaccination of chronically-infected animals treated with lamivudine. J Hepatol. 35:105-11.

2001 Bunch SE, Johnson SE, Cullen JM. Idiopathic noncirrhotic portal hypertension in dogs: 33 cases (1982-1998). J Am Vet Med Assoc, 218:392-9.

2000 Ohashi, K, Marion, P.L., Nakai, H., Meuse, L., Cullen, J.M., Bordier, B.B., Schwall, R., Greenberg, H.B., Glenn, J.S., Kay, M.A., Sustained survival of human hepatocytes in mice: A model for in vivo infection with human hepatitis B and hepatitis D viruses. Nature Med, 6:327-333. 2000 Li, D.H.,Brown, C., Havel.E., Cullen, J.M., Woodchuck lymphotoxin alpha, beta and tumor necrosis factor genes; Structure, characterization and biological activity. Gene,242:295-305.

Lab Personnel

Graduate student: Rachel Cianciolo VMD

Staff: Sashi Gadi