Dean, Gregg, DVM, PhD
Professor of Immunopathology
Director, Center for Comparative Medicine and Translational Research
B.S., Veterinary Science, Colorado State University, 1985
D.V.M., Veterinary Medicine, Colorado State University, 1988
Ph.D., Experimental Pathology, Colorado State University,
1991
Diplomate, American College of Veterinary Pathology
Phone:
919 513 2819
FAX: 919 515-3044
E-mail: gregg_dean@ncsu.edu
Research Interests:
The focus of my research program is the immunopathogenesis and prevention of human and feline immunodeficiency virus infection (HIV and FIV, respectively). FIV is a valuable model of human immunodeficiency virus infection in people and represents a significant health threat to the feline population world-wide. We are investigating the role of innate immune defects induced by HIV/FIV infection. These studies will carefully define the nature of the innate immune defects with an emphasis on toll-like receptors (TLR). Modulation of the innate response through cytokine or TLR manipulation will be addressed as a strategy to reduce debilitating opportunistic infections in AIDS patients. These studies will also determine the impact of a reduced innate immune response on the induction of immunologic memory. Ongoing vaccine studies seek to employ recombinant bacteria as vaccine vectors. We are evaluating recombinant Lactobacillus gasseri and Listeria monocytogenes engineered to express HIV and FIV structural genes and deliver an Env-expressing plasmid to antigen presenting cells as a means to provide effective mucosal and systemic immune responses through oral immunization.
Publications:
Zimmerman DM, Waters WR, Lyashchenko KP, Nonnecke BJ, Armstrong DL, Jacobs WR Jr, Larsen MH, Egan E, Dean GA. Safety and immunogenicity of Mycobacterium tuberculosis {Delta}lysA {Delta}panCD vaccine in domestic cats infected with feline immunodeficiency virus. Clin Vaccine Immunol. 2009 Jan 14. [Epub ahead of print]
Dean, GA. Acute HIV-1 infection: targeting the regulator. Blood, 2008 Oct1;112(7):2600.
Lehman TL, O'Halloran KP, Fallon SA, Habermann LM, Campbell JA, Nordone S, Dean GA, Hoover EA, Avery PR. Altered bone marrow dendritic cell cytokine production to toll-like receptor and CD40 ligation during chronic feline immunodeficiency virus infection. Immunology. 2008 Sep 2. [Epub ahead of print]
Smithberg SR, Fogle JE, Mexas AM, Reckling SK, Lankford SM, Tompkins MB, Dean GA. In vivo depletion of CD4+CD25+ regulatory T cells in cats. Journal of Immunological Methods, 2008 Jan 1;329(1-2):81-91.
Nordone SK, Ignacio GA, Su L, Sempowski GD, Golenbock DT, Li L, Dean GA. Failure of TLR4-driven NFkappa B activation to stimulate virus replication in models of HIV type 1 activation. AIDS Research Human Retroviruses. 2007; Nov;23(11):1387-95.
Lankford S, Petty C, Lavoy A, Reckling S. Tompkins W, Dean GA. Cloning of feline FOXP3 and detectionof expression in CD4+CD25+ regulatory T cells. Veterinary Immunological Immunopathology 2008 Mar 15;122(1-2):159-66.
Yearley JH, Stanton C, Olivry T, Dean GA. Phagocytic plasmacytoma in a dog. Veterinary Clinical Pathology. 2007 Sep;36(3):293-6.
Gupta S, Leutenegger CM, Dean GA, Steckbeck JD, Cole KS, Sparger EE. Vaccination of cats with attenuated feline immunodeficiency virus proviral DNA vaccine expressing gamma interferon. Journal of Virology, 2007; Jan;81(2):465-73.
Nordone, S.K., Ignacio. G.A., Golenbock, D.T., Latz, E., Sempowski, G.D., Su, L., Li, L., Dean, G.A. Toll-like receptor 4 ligation on HIV-1 infected cells does not induce cytokine or virus production. Submitted, 2005.
Dean GA, Lavoy A, Yearley J, Stanton C. Cytokine modulation of the innate immune response in feline immunodeficiency virus-infected cats. Journal of Infectious Disease, 2006; 1;193(11):1520-7.
Gupta S, Dean GA, Leutenneger C, Sparger EE: Construction and characterization of feline immunodeficiency proviral mutants that co-express interferon gamma and green fluorescent protein. AIDS Research and Human Retroviruses, 2006 Apr;22(4):342-9.
Olivry T, LaVoy A, Dunston SM, Brown RS, Lennon M, Warren SJ, Prisayanh P, Muller EJ, Suter MM, Dean GA. Desmolein-1 is a minor autoantigen in dogs with pemphigus foliaceus. Veterinary Immunology and Immunopathology 2006 Apr 15;110(3-4):245-55.
Ignacio, G, Nordone, S., Howard, H.E., Dean, G.A. Toll-like receptor expression in feline lymphoid tissues. Veterinary Immunology & Immunopathology 2005; 106:229-37.
Nordone, SK, Stevens, R, LaVoy, A, Dean G. A. Feline cytokine ELISPOT: issues in assay development. Methods Molecular Biology 2005; 302:167-78
Howard, E,. Fisher I., Dean, G.A., Burkhard, MJ. Methodology for isolation and phenotypic characterization of feline small intestinal leukocytes. Journal of Immunological Methods 2005; 302:36-53
Stevens, R. LaVoy, A., Nordone, S., Burkhard, MJ, Dean, G.A. Construction and characterization of recombinant Listeria monocytogenes as a vaccine vector against feline immunodeficiency virus. Vaccine, 2005, 12:1479-90 .
Dean, G.A , Barger, A., LaVoy, A. Cloning and Expression of feline interleukin 15. Cytokine, 2005, 29:77-83.
Sirriyah J, Dean GA, LaVoy A, Burkhard MJ. Assessment of CD4+ and CD8+ IFN-gamma producing cells by ELISpot in naïve and FIV-infected cats. Veterinary Immunology & Immunopathology, 2004, 102:77-84.
Stevens, R., Howard, K., Nordone, S., Burkhard, MJ, Dean, G.A.. Oral Immunization with recombinant Listeria monocytogenes protects against vaginal challenge with feline immunodeficiency virus. Journal of Virology, 2004, 78(15):8210-8218.
Dean, G.A. , LaVoy, A., Stevens, R., Nordone, S., Burkhard, MJ. Peptide mapping of feline immunodeficiency virus by IFN-g ELISPOT. Veterinary Immunology and Immunopathology, 2004, 100:49-59.
Dean, G.A. , Olivry, T., Stanton, C.A., Pedersen, N.C. In vivo response to experimental feline infectious peritonitis virus infection. Veterinary Microbiology, 97(1-2):1-12, 2003.
Burkhard, M. and Dean, G.A. Transmission and immunopathgenesis of FIV in cats as a model for HIV. Current HIV Research, 1:1:15-29, 2003.
Olivry, T., Dunston, S.M., Rivierre, C., Jackson, H.A., Marcy, K., Peters, E., Dean, G.A. A Randomized Controlled Trial of Misoprostol Monotherapy for Canine Atopic Dermatitis: Effects on Dermal Cellularity and Cutaneous Tumor Necrosis Factor-Alpha. Vet Dermatol, 14(1):37-46, 2003.
Burkhard, M. J., L. Valenski, S. Leavell, G. A. Dean, and W. A. Tompkins. Evaluation of FIV protein-expressing VEE-replicon vaccine vectors in cats. Vaccine 2002; 21:258-68.
Roosje, P. J., G. A. Dean, T. Willemse, V. P. Rutten, and T. Thepen. Interleukin 4-producing CD4+ T cells in the skin of cats with allergic dermatitis. Veterinary Pathology 2002; 39:228-233.
Lockridge, KM, Chien, M, Dean, GA, Cole, KS, Montelaro, RS, Luciw, PA, Sparger, EE. Protective Immunity Against Feline Immunoeficiency Virus Induced by Inoculation with vif-deleted Proviral DNA. Virology, 273(1):67-79, 2000.
Neel, J. and Dean, G.A., A Mass In The Spinal Column Of A Dog [Nephroblastoma]. Veterinary Clinical Pathology, 3:87-92, 2000.
Woo, JC, Dean, GA, Lavoy, A, Clark, R and Moore, PF. Investigation of recombinant human insulin-like growth factor type I in thymus regeneration in the acute stage of experimental FIV infection in juvenile cats. AIDS Research and Human Retroviruses, 15:1377-1388, 1999.
Dean, G.A , Sparger, E.E, and Himathongkham, S. Virologic And Biologic Characterization Of Feline Immunodeficiency Virus Molecular Clones, In Vivo. Journal of Virology, 73:2596-2603, 1999.
Olivry, T., Dean, G.A., Tompkins, M.B., Dow, J., and Moore, P.F. Amplification Of Cytokine Gene Transcripts In The Skin Of Dogs With Atopic Dermatitis. Experimental Dermatology, 8:204-211, 1999.
Dean, G.A. , Bernales, J., Pedersen, N.C. Effect Of Feline Immunodeficiency Virus On Cytokine Response To Listeria Monocytogenes In Vivo. Veterinary Immunology and Immunopathology, 65:125-38, 1998.
Dean, G.A. and Pedersen, N.C. Cytokine Response In Multiple Lymphoid Tissues During The Primary Phase Of Feline Immunodeficiency Virus Infection. Journal of Virology, 72:9436-40.
Elder, J.H., Dean, G.A., Hoover, E.A., Hoxie, J.A., Malim, M.H., Neil, J.C., North, T.W., Tompkins, M.B., Tompkins, W.A.F., Pedersen, N.C., and Miller, R.H. Lessons From The Cat: Use Of Feline Immunodeficiency Virus As A Means To Develop Intervention Stragies Effective Against Human Immunodeficiency Virus Type 1. AIDS and Human Retroviruses, 14:797-801, 1998.
Dean, G.A. , Higgins, J., LaVoy, A., Fan, Z., Pedersen, N.C. Measurement Of Feline Cytokine Gene Expression By Quantitative-Competitive RT-PCR. Veterinary Immunology and Immunopathology, 63:73-82, 1998.
Pedersen, N.C., Dean, G.A., Bernales, J., Sukura, A., and Higgins, J. Listeria Monocytogenes And Serratia Marcescens Infections As Models For Th1/Th2 Immunity In Laboratory Cats. Veterinary Immunology and Immunopathology, 63:83-103, 1998.
Fehr, D., Dean G.A., Huder, J., Fan, Z., Huettner, S., Higgins, J.W., Pedersen, N.C., Lutz, H. Nucleotide And Predicted Peptide Sequence Of Feline Interleukin-12. The Journal of Sequencing and Mapping, 8:77-82, 1997.
Woo, J.C., Dean, G.A., Pedersen, N.C., Moore, P.F. Pathologic And Immunopathologic Changes In The Thymus During The Acute Stage Of Experimentally-Induced Feline Immunodeficiency Virus Infection. Journal of Virology, 71:8632-8641, 1997.
Dean, G.A. , Reubel, G.H., and Pedersen, N.C. SIV Infection of CD8+ Lymphocytes In Vivo. Journal of Virology, 70:5646-5650, 1996.
Quackenbush, S.L., Dean, G.A., Mullins, J.I., Hoover, E.A. Analysis of FeLV-FAIDS Provirus Burden and Productive Infection in Lymphocyte Subsets in Vivo. Virology, 223:1-9, 1996.
Dean, G.A. , Reubel, G.H., Moore, P.F., and Pedersen, N.C. Quantification of Proviral Feline Immunodeficiency Virus in Lymphocyte Subsets of Blood and Lymph Node. Journal of Virology, 70:5165-5169, 1996.
Dean, G.A. , Groshek, P.M., Hoover, E.A. Immunophenotypic Analysis of Feline Haemolymphatic Neoplasia Using Flow Cytometry. Comparative Hematology International, 5:84-92, 1995.
Groshek, P.M., Dean, G.A., Hoover, E.A. Monoclonal Antibodies Identifying Feline Hemopoietic Cell Lineages. Comparative Hematology International, 4:181-191, 1994.
Rimstad, E., Reubel, G.H., Dean, G.A., Higgins, J., and Pedersen, N.C. Cloning, Expression and Characterization of Biologically Active Feline Tumor Necrosis Factor a. Veterinary Immunology and Immunopathology, 45:297-310, 1995.
Reubel, G.H., Dean, G.A., George, J.W., Barlough, J.E., Pedersen, N.C. Effects of Incidental Infections and Immune-activation on Disease Progression in Experimentally Feline Immunodeficiency Virus Infected Cats. Journal of Acquired Immunodeficiency Virus, 7:1003-1015, 1994.
Israel, Z.R., Dean, G.A., Maul, D.H., O'Neil, S.P., Dreitz, M.J., Mullins, J.I., Fultz, P.N., Hoover, E.A. Early Pathogenesis of Disease Caused by SIVsmmPBj14 Molecular Clone 1.9 in Macaques. AIDS Research and Human Retroviruses, 3:277-285, 1993.
Dean, G.A. , Groshek, P.M., Mullins, J.I., Hoover, E.A. Hematopoietic Target Cells of Anemogenic Subgrop C versus Nonanemogenic Subgroup A Feline Leukemia Virus. Journal of Virology, 66(9):5561-5568, 1992.
Dean, G.A. , Quackenbush, S.L., Ackley, C.D., Cooper, M.D., Hoover, E.A. Flow Cytometric Analysis of T-Lymphocyte Subsets in Cats. Veterinary Immunology and Immunopathology, 28:327-335, 1991.
Quackenbush, S.L., Donahue, P.R., Dean, G.A., Myles, M.H., Ackley, C.D., Cooper, M.D., Mullins, J.I., Hoover, E.A. Lymphocyte Subset Alterations and Viral Determinants of Immunodeficiency Disease Induction by the Feline Leukemia Virus FeLV-FAIDS. Journal of Virology, 64 (11): 5465-5474, 1990.
LAB PERSONNEL
Alora LaVoy, Research Specialist
alora_lavoy@ncsu.edu
Julie Long, Research Assistant
julie_long@ncsu.edu
Lin Zhang, Research Assistant
lzhang11@ncsu.edu
Graduate Students:
Sara Gunderson sabumgar@ncsu.edu
Rochelle Mikkelsen rochelle.mikkelsen@gmail.com
Rita de Cassia Simoes rdsimoes@ncsu.edu
Laura Stoeker llstoeke@ncsu.edu