Skip to main content
 

Profile

John Cullen, VMD, PhD, DACVP, FIATP

Professor, Anatomic Pathology

Contact:

jcullen@ncsu.edu
Office: 919.513.7720
Lab: Histology (Biopsy)
Lab Phone: 919.513.6390

Course Involvement
VMP 931: General Pathology
VMP 941: Systemic Pathology
VMP 991D: Necropsy Pathology Selective
VMP 977: Clinical Laboratory/Necropsy
VMP 991M: Surgical Pathology Selective
VMM 991K: Selective in Nondomestic Animal Pathology
CBS 804: Seminar in Necropsy Pathology
CBS 803: Seminar in Surgical Pathology
CBS 810F: Pathology Concentration Seminar
Graduate Courses

CBS 565: Fundamentals of Biomedical Sciences
----
I have been at NCSU CVM for more than 30 years active in teaching, service and research. My early training occurred at the University of Pennsylvania for undergarduate and veterinary school. After a small animal internship at Angell Memorial Animal Hospital and a brief stint as a small animal practitioner I entered a combined residency/PhD program at UC Davis. I have been at NCSU ever since working in various aspects of liver disease, both spontaneous and experimental. Animal models of viral hepatitis have been my main emphasis over the last number of years.
Affiliations
AVMA, ACVP, AASLD
Certifications
Diplomate, American College of Veterinary Pathology
Fellow, International Academy of Toxicologic Pathologists
VMD, University of Pennsylvania
PhD, University of California at Davis
Diplomate: American College of Veterinary Pathology
Fellow: International Academy of Toxicologic Pathologists
----
Dip. ACVP
Infectious Diseases, Spontaneous Animal Disease Models
Research Area: drug-induced hepatic disease, animal models of viral hepatitis

Current Research: Animal Models of Hepatitis C virus and Hepatic toxicity with the Comparisons of acute hepatotoxicity in conventional and germ free mice, Acute biliary toxicity in IL-10 k.o. mice, Acute biliary toxicity in Kupffer cell depleted mice, and Acute gene expression in biliary epithelia following oral ANIT exposure
----
My focus is diseases of the liver. I have worked primarily with models of viral hepatitis, but am also engaged in studies of hepatic toxicity.
  • Development of an animal model for radiofrequency ablation of primary, virally induced hepatocellular carcinoma in the woodchuck.Burke CT, Cullen JM, State A, Gadi S, Wilber K, Rosenthal M, Bulysheva A, Pease A, Mauro MA, Fuchs H. | J Vasc Interv Radiol. 22:1613-1618
  • Molecular cytogenetic characterization of canine histiocytic sarcoma: A spontaneous model for human histiocytic cancer identifies deletion of tumor suppressor genes and highlights influence of genetic background on tumor behavior.Hedan B, Thomas R, Motsinger-Reif A, Abadie J, Andre C, Cullen J, Breen M. | BMC Cancer. 26;11:201.
  • H-ras consensus sequence and mutations in primary hepatocellular carcinomas of lemurs and lorises.Cullen JM, Williams C, Zadrozny L, Otstot JT, Solomon GG, Sills RC, Hong HH. | Vet Pathol. 48:868-74.
  • Acute ANIT Toxicity in Male IL-10 Knockout and Wild Type MiceFaiola B, Peterson R, Jordan, H, Kimbrough CA, Cullen JM. | Tox Pathol (In press)
  • Time Course Gene Expression using Laser Capture Microscopy-extracted Bile Ducts, But Not Hepatic Parenchyma, Reveals Acute Alpha-Napthylisothiocyante Toxicity.Cullen J M, Falls J G, Brown HR, Yoon LW, Cariello N F, Faiola B, Kimbrough C L, Jordan HL, Miller R T. | Tox Pathol (In press)
  • Spontaneous Hepatocellular Carcinoma in Captive Prosimians..Zadrozny LM, Williams CV, Remick AK, Cullen JM | Vet Pathol. 2010 Feb
  • Congenital hepatic fibrosis in 5 dogs.Brown DL, Van Winkle T, Cecere T, Rushton S, Brachelente C, Cullen JM. | Vet Pathol. 47:102-7.
  • Summary of the world small animal veterinary association standardization committee guide to classification of liver disease in dogs and cats.Cullen JM. | Vet Clin North Am Small Anim Pract. 39:395-418
  • Aflatoxin B1 and/or hepatitis B virus induced tumor spectrum in a genetically engineered hepatitis B virus expression and Trp53 haploinsufficient mouse model system for hepatocarcinogenesis.Cullen JM, Brown DL, Kissling GE, Foley JF, Rizzo J, Marion PL, Parron VI, French JE. | Toxicol Pathol. 37:333-42.
  • Serologic survey of woodchuck hepatitis virus in North Carolina woodchucks (Marmota monax).Cullen JM, Lindsey-Pegram D, Cote PJ. | J Zoo Wildl Med. 39:263-5
  • Harderian gland neoplasms in captive, wild-caught Beechey ground squirrels (Spermophilus beecheyi).Ranck RS, Cullen JM, Waggie KS, Marion PL | Vet Pathol. 45:388-92
  • Expression of CDK4 or CDK2 in mouse oral cavity is retained in adult pituitary with distinct effects on tumorigenesis.Macias E, de Marval PL, Senderowicz A, Cullen J, Rodriguez-Puebla ML. | Cancer Res. 68:162-71
  • Mesenchymal hamartoma of the liver in a late-term equine fetus..Brown DL, Anderson M, Cullen JM,   | Vet Pathol. 44:100-2
  • Fall panicum (Panicum dichotomiflorum) hepatotoxicosis in horses and sheep.Johnson AL, Divers TJ, Freckleton ML, McKenzie HC, Mitchell E, Cullen JM, McDonough SP. | J Vet Intern Med. 20:1414-21
  • The role of pathology in the identification of drug-induced hepatic toxicityCullen J M    Miller R T, | Expert Opinion on Drug Metabolism & Toxicology Vol. 2, No. 2, Pgs 241-247
  • Mechanistic classification of liver injury.Cullen JM. | Toxicol Pathol. 33: 6-8.
  • cDNA microarray analysis of HBV transgenic mouse liver identifies genes in lipid biosynthetic and growth control pathways affected by HBV.Hajjou M, Norel R, Carver R, Marion P, Cullen J, Rogler LE, Rogler CE. | J, Med Virol. 77: 57-65.
  • Paraneoplastic pemphigus in a dog with splenic sarcoma.Elmore SA, Basseches J, Anhalt GJ, Cullen JM, Olivry T. | Vet Pathol. 42: 88-91.
  • Adenovirus-based gene therapy during clevudine treatment of woodchucks chronically infected with woodchuck hepatitis virus.Zhu Y, Cullen JM, Aldrich CE, Saputelli J, Miller D, Seeger C, Mason WS, Jilbert AR. | Virol. 327:26-40.
  • Woodchuck interleukine-6 gene: structure, characterization, and biologic activity.Li DH, Kumanogoh A, Cao TM, Parnes JR, Cullen JM. | Gene. 342: 157-164.
  • Antiviral Efficacy and Pharmacokinetics of Oral Adefovir Dipivoxil in Chronically WHV-infected Woodchucks.Cullen JM, Li DH, Brown C, Eisenberg GC, Wolfe KC, Toole J, Gibbs C | Antimicrob Agents Chemother. 45:2740-5
  • Kinetics of hepadnavirus loss from the liver during inhibition of viral DNA synthesis.Zhu, Y., Yamamoto, T., Cullen, J., Saputelli, J., Aldrich C.E., Miller, D.S., Litwin, S., Furman, P.A., Jilbert, A.R., Mason, W.S. | J Virol, 75:311-322.
  • T-helper cell response to woodchuck hepatitis virus antigens after therapeutic vaccination of chronically-infected animals treated with lamivudine.Hervas-Stubbs S, Lasarte JJ, Sarobe P, Vivas I, Condreay L, Cullen JM, Prieto J,Borras-Cuesta F | J Hepatol. 35:105-11.
  • Idiopathic noncirrhotic portal hypertension in dogs: 33 cases (1982-1998).Bunch SE, Johnson SE, Cullen JM. | J Am Vet Med Assoc, 218:392-9.
  • Sustained survival of human hepatocytes in mice: A model for in vivo infection with human hepatitis B and hepatitis D viruses.Ohashi, K, Marion, P.L., Nakai, H., Meuse, L., Cullen, J.M., Bordier, B.B., Schwall, R., Greenberg, H.B., Glenn, J.S., Kay, M.A., | Nature Med, 6:327-333.
  • Woodchuck lymphotoxin alpha, beta and tumor necrosis factor genes; Structure, characterization and biological activity.Li, D.H.,Brown, C., Havel.E., Cullen, J.M., | Gene, 242:295-305.
  • Lack of association of ABCB4 insertion mutation with gallbladder mucoceles in dogs.John M. Cullen, Cynthia J. Willson, Jonathan D. Minch, Carie L. Kimbrough, Katrina L. Mealey,   | J Vet Diag Invest (In Press)
  • Mechanistic Interpretation of Elevated Liver Biomarkers in Beagle Dogs Treated with GABAA Receptor Antagonist NP260 in the Absence of Histopathological Correlate.Alison H. Harrill, John S. Eaddy, Kelly Rose, John M. Cullen, Lakshmi Ramanathan, Stephen Wanaski, Stephen Collins, Yu Ho, Paul B. Watkins, Edward L. LeCluyse,    | Tox App Pharm (In Press)
  • Role of pregnane X receptor in obesity and glucose homeostasis in male miceSpruiell K, Richardson RM, Cullen JM, Awumey EM, Gonzalez FJ, Gyamfi MA,   | J Biol Chem. 289:3244-61. 289(6):3244-61
  • Dietary fructose induces endotoxemia and hepatic injury in calorically controlled primates.Kavanagh K, Wylie AT, Tucker KL, Hamp TJ, Gharaibeh RZ, Fodor AA, Cullen JM. | Am J Clin Nutr. 2013 98:349-57.
  • Biliary Proliferative Lesions in the Sprague-Dawley Rat: Adverse/Non-adverse.Hailey JR, Nold JB, Brown RH, Cullen JM, Holder JC, Jordan HL, Ennulat D, Miller RT. | Toxicol Pathol. Aug 19. [Epub ahead of print]
  • Gene Expression Analysis and Urinary Biomarker Assays Reveal Activation of Tubulointerstitial Injury Pathways in a Rodent Model of Chronic Proteinuria (Doxorubicin Nephropathy),Cianciolo R., Yoon L., Krull D., Stokes A., Jordan H., Rodriguez A., Davis C., Falls J., Cullen J., Kimbrough C., Berridge B. | Nephron Experimental 124:1-10.
  • Identification of a pegivirus (GBV-like virus) that infects horses.Kapoor A, Simmonds P, Cullen JM, Scheel T, Medina JL, Giannitti F, Nishiuchi E, Brock KV, Burbelo PD, Rice CM, Lipkin WI. | J Virol. 87:185-90.
  • Identification of rodent homologs of hepatitis C virus and pegiviruses.Kapoor A, Simmonds P, Scheel TK, Hjelle B, Cullen JM, Burbelo PD, Chauhan LV, Duraisamy R, Sanchez Leon M, Jain K, Vandegrift KJ, Calisher CH, Rice CM, Lipkin WI. | MBio. Apr 9;4(2). E00216
  • Chikungunya Virus Host-Range E2-Transmembrane Deletion Mutants Induce Protective Immunity Against Challenge in C57BL/6J Mice.Piper A, Ribeiro M, Smith KM, Briggs CM, Huitt E, Nanda K, Spears CJ, Quiles M, Cullen J, Thomas ME, Brown DT, Hernandez R. | J Virol. Apr 3. [Epub ahead of print]
  • Cardiac structure and function in female carriers of a canine model of Duchenne muscular dystrophy.Kane AM, Defrancesco TC, Boyle MC, Malarkey DE, Ritchey JW, Atkins CE, Cullen JM, Kornegay JN, Keene BW. | Res Vet Sci. Jun;94(3):610-7
  • Diode laser endoscopic cyclophotocoagulation in the normal equine eye.Harrington JT, McMullen RJ Jr, Cullen JM, Campbell NB, Gilger BC. | Vet Ophthalmol. 16:97-110.
  • Limited yield of diagnoses of intrahepatic infectious causes of canine granulomatous hepatitis from archival liver tissue.Hutchins RG, Breitschwerdt EB, Cullen JM, Bissett SA, Gookin JL. | J Vet Diagn Invest. Sep;24(5):888-94
  • Evaluation of diode endoscopic cyclophotocoagulation in bovine cadaver eyes.Harrington JT, McMullen RJ Jr, Cullen JM, Gilger BC | Am J Vet Res. 73:1445-52.
  • Reye's or Reye's-like syndrome in western lowland gorilla (Gorilla gorilla gorilla).Minter LJ, Cullen JM, Loomis MR. | J Med Primatol. 41:329-31.
  • Serology-enabled discovery of genetically diverse hepaciviruses in a new host.Burbelo PD, Dubovi EJ, Simmonds P, Medina JL, Henriquez JA, Mishra N, Wagner J, Tokarz R, Cullen JM, Iadarola MJ, Rice CM, Lipkin WI, Kapoor A. | J Virol. 86:6171-8.
  • Effects of Kupffer Cell Depletion on Acute Alpha-Napthylisothiocyanate-induced Liver Toxicity in Male Mice.Cullen JM, Faiola B, Melich DH, Peterson RA, Jordan HL, Kimbrough CL, Prescott JS, Miller RT. | Toxicol Pathol. Aug 10.
  • Limited yield of diagnoses of intrahepatic infectious causes of canine granulomatous hepatitis from archival liver tissue.  Hutchins RG, Breitschwerdt EB, Cullen JM, Bissett SA, Gookin JL. | J Vet Diagn Invest.; 24:888-94
  • Time Course Gene Expression using Laser Capture Microscopy-extracted Bile Ducts, But Not Hepatic Parenchyma, Reveals Acute Alpha-Napthylisothiocyante Toxicity.Cullen J M, Falls J G, Brown HR, Yoon LW, Cariello N F, Faiola B, Kimbrough C L, Jordan HL, Miller R T. | Tox Pathol (In press)
  • Spontaneous Hepatocellular Carcinoma in Captive Prosimians..Zadrozny LM, Williams CV, Remick AK, Cullen JM | Vet Pathol. 2010 Feb
  • Congenital hepatic fibrosis in 5 dogs.Brown DL, Van Winkle T, Cecere T, Rushton S, Brachelente C, Cullen JM. | Vet Pathol. 47:102-7.
  • Summary of the world small animal veterinary association standardization committee guide to classification of liver disease in dogs and cats.Cullen JM. | Vet Clin North Am Small Anim Pract. 39:395-418
  • Aflatoxin B1 and/or hepatitis B virus induced tumor spectrum in a genetically engineered hepatitis B virus expression and Trp53 haploinsufficient mouse model system for hepatocarcinogenesis.Cullen JM, Brown DL, Kissling GE, Foley JF, Rizzo J, Marion PL, Parron VI, French JE. | Toxicol Pathol. 37:333-42.
  • Serologic survey of woodchuck hepatitis virus in North Carolina woodchucks (Marmota monax).Cullen JM, Lindsey-Pegram D, Cote PJ. | J Zoo Wildl Med. 39:263-5
  • Harderian gland neoplasms in captive, wild-caught Beechey ground squirrels (Spermophilus beecheyi).Ranck RS, Cullen JM, Waggie KS, Marion PL | Vet Pathol. 45:388-92
  • Expression of CDK4 or CDK2 in mouse oral cavity is retained in adult pituitary with distinct effects on tumorigenesis.Macias E, de Marval PL, Senderowicz A, Cullen J, Rodriguez-Puebla ML. | Cancer Res. 68:162-71
  • Mesenchymal hamartoma of the liver in a late-term equine fetus.. | Vet Pathol. 44:100-2
  • Selective deletion of antigen-specific CD8+ T cells by MHC class I tetramers coupled to the type I ribosome-inactivating protein saporin.Hess PR, Barnes C, Woolard MD, Johnson MD, Cullen JM, Collins EJ, Frelinger JA. | 15:3300-7
  • Fall panicum (Panicum dichotomiflorum) hepatotoxicosis in horses and sheep.Johnson AL, Divers TJ, Freckleton ML, McKenzie HC, Mitchell E, Cullen JM, McDonough SP. | J Vet Intern Med. 20:1414-21
  • The role of pathology in the identification of drug-induced hepatic toxicity | Expert Opinion on Drug Metabolism & Toxicology Vol. 2, No. 2, Pgs 241-247
  • Mechanistic classification of liver injury.Cullen JM. | Toxicol Pathol. 33: 6-8.
  • cDNA microarray analysis of HBV transgenic mouse liver identifies genes in lipid biosynthetic and growth control pathways affected by HBV.Hajjou M, Norel R, Carver R, Marion P, Cullen J, Rogler LE, Rogler CE. | J, Med Virol. 77: 57-65.
  • Paraneoplastic pemphigus in a dog with splenic sarcoma.Elmore SA, Basseches J, Anhalt GJ, Cullen JM, Olivry T. | Vet Pathol. 42: 88-91.
  • Adenovirus-based gene therapy during clevudine treatment of woodchucks chronically infected with woodchuck hepatitis virus.Zhu Y, Cullen JM, Aldrich CE, Saputelli J, Miller D, Seeger C, Mason WS, Jilbert AR. | Virol. 327:26-40.
  • Woodchuck interleukine-6 gene: structure, characterization, and biologic activity.Li DH, Kumanogoh A, Cao TM, Parnes JR, Cullen JM. | Gene. 342: 157-164.
  • Antiviral Efficacy and Pharmacokinetics of Oral Adefovir Dipivoxil in Chronically WHV-infected Woodchucks.Cullen JM, Li DH, Brown C, Eisenberg GC, Wolfe KC, Toole J, Gibbs C | Antimicrob Agents Chemother. 45:2740-5
  • Kinetics of hepadnavirus loss from the liver during inhibition of viral DNA synthesis.Zhu, Y., Yamamoto, T., Cullen, J., Saputelli, J., Aldrich C.E., Miller, D.S., Litwin, S., Furman, P.A., Jilbert, A.R., Mason, W.S. | J Virol, 75:311-322.
  • T-helper cell response to woodchuck hepatitis virus antigens after therapeutic vaccination of chronically-infected animals treated with lamivudine.Hervas-Stubbs S, Lasarte JJ, Sarobe P, Vivas I, Condreay L, Cullen JM, Prieto J,Borras-Cuesta F | J Hepatol. 35:105-11.
  • Idiopathic noncirrhotic portal hypertension in dogs: 33 cases (1982-1998).Bunch SE, Johnson SE, Cullen JM. | J Am Vet Med Assoc, 218:392-9.
  • Sustained survival of human hepatocytes in mice: A model for in vivo infection with human hepatitis B and hepatitis D viruses.Ohashi, K, Marion, P.L., Nakai, H., Meuse, L., Cullen, J.M., Bordier, B.B., Schwall, R., Greenberg, H.B., Glenn, J.S., Kay, M.A., | Nature Med, 6:327-333.
  • Woodchuck lymphotoxin alpha, beta and tumor necrosis factor genes; Structure, characterization and biological activity.Li, D.H.,Brown, C., Havel.E., Cullen, J.M., | Gene, 242:295-305.
  • Lack of association of ABCB4 insertion mutation with gallbladder mucoceles in dogs. | J Vet Diag Invest (In Press)
  • Mechanistic Interpretation of Elevated Liver Biomarkers in Beagle Dogs Treated with GABAA Receptor Antagonist NP260 in the Absence of Histopathological Correlate. | Tox App Pharm (In Press)
  • Role of pregnane X receptor in obesity and glucose homeostasis in male mice | J Biol Chem. 289:3244-61. 289(6):3244-61
  • Dietary fructose induces endotoxemia and hepatic injury in calorically controlled primates.Kavanagh K, Wylie AT, Tucker KL, Hamp TJ, Gharaibeh RZ, Fodor AA, Cullen JM. | Am J Clin Nutr. 2013 98:349-57.
  • Biliary Proliferative Lesions in the Sprague-Dawley Rat: Adverse/Non-adverse.Hailey JR, Nold JB, Brown RH, Cullen JM, Holder JC, Jordan HL, Ennulat D, Miller RT. | Toxicol Pathol. Aug 19. [Epub ahead of print]
  • Gene Expression Analysis and Urinary Biomarker Assays Reveal Activation of Tubulointerstitial Injury Pathways in a Rodent Model of Chronic Proteinuria (Doxorubicin Nephropathy),Cianciolo R., Yoon L., Krull D., Stokes A., Jordan H., Rodriguez A., Davis C., Falls J., Cullen J., Kimbrough C., Berridge B. | Nephron Experimental 124:1-10.
  • Identification of a pegivirus (GBV-like virus) that infects horses.Kapoor A, Simmonds P, Cullen JM, Scheel T, Medina JL, Giannitti F, Nishiuchi E, Brock KV, Burbelo PD, Rice CM, Lipkin WI. | J Virol. 87:185-90.
  • Identification of rodent homologs of hepatitis C virus and pegiviruses.Kapoor A, Simmonds P, Scheel TK, Hjelle B, Cullen JM, Burbelo PD, Chauhan LV, Duraisamy R, Sanchez Leon M, Jain K, Vandegrift KJ, Calisher CH, Rice CM, Lipkin WI. | MBio. Apr 9;4(2). E00216
  • Chikungunya Virus Host-Range E2-Transmembrane Deletion Mutants Induce Protective Immunity Against Challenge in C57BL/6J Mice.Piper A, Ribeiro M, Smith KM, Briggs CM, Huitt E, Nanda K, Spears CJ, Quiles M, Cullen J, Thomas ME, Brown DT, Hernandez R. | J Virol. Apr 3. [Epub ahead of print]
  • Cardiac structure and function in female carriers of a canine model of Duchenne muscular dystrophy.Kane AM, Defrancesco TC, Boyle MC, Malarkey DE, Ritchey JW, Atkins CE, Cullen JM, Kornegay JN, Keene BW. | Res Vet Sci. Jun;94(3):610-7
  • Diode laser endoscopic cyclophotocoagulation in the normal equine eye.Harrington JT, McMullen RJ Jr, Cullen JM, Campbell NB, Gilger BC. | Vet Ophthalmol. 16:97-110.
  • Limited yield of diagnoses of intrahepatic infectious causes of canine granulomatous hepatitis from archival liver tissue.Hutchins RG, Breitschwerdt EB, Cullen JM, Bissett SA, Gookin JL. | J Vet Diagn Invest. Sep;24(5):888-94
  • Evaluation of diode endoscopic cyclophotocoagulation in bovine cadaver eyes.Harrington JT, McMullen RJ Jr, Cullen JM, Gilger BC | Am J Vet Res. 73:1445-52.
  • Reye's or Reye's-like syndrome in western lowland gorilla (Gorilla gorilla gorilla).Minter LJ, Cullen JM, Loomis MR. | J Med Primatol. 41:329-31.
  • Serology-enabled discovery of genetically diverse hepaciviruses in a new host.Burbelo PD, Dubovi EJ, Simmonds P, Medina JL, Henriquez JA, Mishra N, Wagner J, Tokarz R, Cullen JM, Iadarola MJ, Rice CM, Lipkin WI, Kapoor A. | J Virol. 86:6171-8.
  • Effects of Kupffer Cell Depletion on Acute Alpha-Napthylisothiocyanate-induced Liver Toxicity in Male Mice.Cullen JM, Faiola B, Melich DH, Peterson RA, Jordan HL, Kimbrough CL, Prescott JS, Miller RT. | Toxicol Pathol. Aug 10.
  • Development of an animal model for radiofrequency ablation of primary, virally induced hepatocellular carcinoma in the woodchuck.Burke CT, Cullen JM, State A, Gadi S, Wilber K, Rosenthal M, Bulysheva A, Pease A, Mauro MA, Fuchs H. | J Vasc Interv Radiol. 22:1613-1618
  • Molecular cytogenetic characterization of canine histiocytic sarcoma: A spontaneous model for human histiocytic cancer identifies deletion of tumor suppressor genes and highlights influence of genetic background on tumor behavior.Hedan B, Thomas R, Motsinger-Reif A, Abadie J, Andre C, Cullen J, Breen M. | BMC Cancer. 26;11:201.
  • H-ras consensus sequence and mutations in primary hepatocellular carcinomas of lemurs and lorises.Cullen JM, Williams C, Zadrozny L, Otstot JT, Solomon GG, Sills RC, Hong HH. | Vet Pathol. 48:868-74.
  • Acute ANIT Toxicity in Male IL-10 Knockout and Wild Type MiceFaiola B, Peterson R, Jordan, H, Kimbrough CA, Cullen JM. | Tox Pathol (In press)