Description: Organs are dynamic, complex and coordinated networks of cells. To understand organ function in normal and diseased states, their anatomy, including transcriptional profile and intracellular pathways, needs to be captured as a whole. Tissue clearing and labeling methods were devised to render entire organs transparent by removing lipids, allowing light penetration throughout. Cleared tissue facilitates the 3D structural mapping and connectivity of whole organs with unprecedented resolution and detail. Here we will discuss the technical challenges (e.g. imaging throughput and data analysis) and solutions towards anatomical and transcriptional profiling of intact organs.
Location: RB101, CVM, NC State University