A team led by researchers from North Carolina State University has developed two new approaches for incorporating antimicrobial properties into microneedles—vanishingly thin needles that hold great promise for use in portable medical devices. Researchers expect the findings to spur development of new medical applications using microneedles.
Microneedles cause less pain, tissue damage and skin inflammation for patients, and could be a significant component of portable medical devices for patients with chronic conditions, such as Parkinson’s disease or diabetes. However, longstanding concerns regarding the possibility of infection associated with microneedles have been an obstacle to their widespread adoption – until now.
[Dr. Nancy Monteiro-Riviere, professor of investigative dermatology and toxicology with the College of Veterinary Medicine’s Center for Chemical Toxicology Research and Pharmacokinetics, was one of the study’s co-authors.]
The first new technique is for use with microneedles that would be incorporated into permanent or semi-permanent medical devices – such as glucose monitors for patients with diabetes. The researchers found that modifying the surface of a microneedle with an antimicrobial coating both prevented microbial growth and did not adversely affect skin cell growth. Researchers applied the coating using a laser-based vapor deposition process that created a thin film of silver (which is antimicrobial) on the microneedle surface.
The second approach is applicable to degradable microneedles, which are designed to dissolve on the skin surface and can be used for single-use drug delivery situations such as vaccine delivery. This technique involves incorporating an antimicrobial agent into the material used to make the microneedle itself. As the degradable microneedle dissolves it releases the antimicrobial agent, guarding against infection.
“We expect these findings to result in more widespread use of microneedles in outpatient treatments and technologies,” says Dr. Roger Narayan, lead author of the research. “For example, microneedles could be used as a relatively pain-free and user-friendly alternative to conventional needles in diabetes treatment. They may also figure into new technologies pertaining to the delivery of anti-cancer drugs.” Narayan is a professor in the joint biomedical engineering department of NC State’s College of Engineering and the University of North Carolina at Chapel Hill.
The research, “Two Photon Polymerization Of Microneedles For Transdermal Drug Delivery,” will be presented May 24 at the First International Conference On Microneedles in Atlanta. The work was funded by the National Science Foundation and the National Institutes of Health. The research was co-authored by Dr. Nancy Monteiro-Riviere, professor of investigative dermatology and toxicology at the Center for Chemical Toxicology Research and Pharmacokinetics at NC State, as well as researchers from North Dakota State University, Laser Zentrum Hannover and other institutions.
Microneedles cause less pain, tissue damage and skin inflammation for patients. This image is a scanning electron micrograph showing an array of biodegradable polyethylene glycol-based microneedles with antimicrobial properties.
Note to editors: The study abstract follows.
“Two Photon Polymerization Of Microneedles For Transdermal Drug Delivery”
Authors: Roger J. Narayan, Nancy A. Monteiro-Riviere, North Carolina State University; Bret Chisholm, Shane Stafslien, North Dakota State University; Boris Chichkov, Aleksandr Ovsianikov, Laser Zentrum Hannover; et al.
Presented: May 24, 2010, First International Conference On Microneedles, Atlanta
Abstract: Two-photon polymerization is a laser-based process that may be used to fabricate microneedles and other microscale devices for transdermal drug delivery. In two photon polymerization, femtosecond pulses from a titanium: sapphire laser are used to selectively polymerize a photosensitive material; this technology has previously been used to fabricate scaffolds for regenerative medicine. We have used two photon polymerization to fabricate hollow microneedles with in-plane and out-of-plane geometries; these microneedles have been examined using porcine skin. A combination of two photon polymerization and polydimethylsiloxane micromolding has been used to fabricate solid microneedles out of commercially available photopolymers. There are concerns regarding the risk of infection with microneedle use; microneedle-created pores in the stratum corneum layer of the epidermis could enable pathogenic microorganisms (e.g., Staphylococcus aureus) to enter the body. Antimicrobial microneedles have been prepared using composite materials that contain antimicrobial agents (e.g., gentamicin sulfate) and biocompatible polymers (e.g., polyethylene glycol). In addition, microneedles have been coated with thin films of antimicrobial materials (e.g., silver) using a room temperature physical vapor deposition process known as pulsed laser deposition. Our results suggest that laser-based processes, including two photon polymerization and two photon polymerization-micromolding, may be used to prepare microneedles as well as other functional microscale devices with unique drug delivery capabilities.
Posted May 20, 2010