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Research Lab – Department of Clinical Sciences 

Leukocyte Biology

The Leukocyte Biology Laboratory is interested in understanding the molecular mechanisms of phagocyte activation. Specifically, we are studying the role of cytoskeletal proteins such as the leukocyte specific actin bundling protein L-plastin (LPL), in regulating the signaling cascades which lead to the development of the effector phenotype in neutrophils and macrophages. 

NC State CVM Horse feeding in pasture

 

We are defining the role of LPL and the actin cytoskeleton in regulating signal transduction pathways during integrin activation and the role of beta 2 integrin (CD11/CD18 complex) activation in adhesion, respiratory burst and reactive oxygen production, degranulation, prostaglandin and leukotriene production, migration, and phagocytosis. We are applying this information to clinically important inflammatory diseases, particularly gastrointestinal inflammation and arthritis. For example, we are studying the role of neutrophils in mucosal injury during gastrointestinal inflammation induced by ischemic injury and the mechanism of neutrophil and macrophage activation in immune complex disease such as rheumatoid arthritis. We are also interested in the strategies used by intracellular bacterial gastrointestinal pathogens such as Salmonella, to invade macrophages, modulate their activation, induce apoptosis, and subsequently evade the host immune response.

Our laboratory collaborates extensively with Anthony Blikslager and the Gastrointestinal Physiology Laboratory, and the Center for Gastrointestinal Biology and Disease at UNC-Chapel Hill.

 

Resources 

  • Expression of cyclooxygenase-1 and -2 in naturally occurring squamous cell carcinomas in horsesElce YA, Orsini JA, Blikslager AT | Am J Vet Res 2007 Jan;68(1):76-80. PubMed PMID: 17199422
  • Functional cooperation between Fc receptors and complement receptors in phagocytesJones SL, Brown EJ | In: van de Winkel JGJ, Capel PJA (eds) Human IgG Fc Receptors RG Landes Co, Georgetown, Texas, 1996
  • (1998) Two signaling mechanisms for activation of alphaMbeta2 avidity in polymorphonuclear neutrophils.Jones SL, Knaus UG, Bokoch GM, Brown EJ. | J Biol Chem 273:10556-10566.
  • (1996) FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils.Jones SL, Brown EJ. | J Biol Chem 271:14623-14630.
  • (2002) The effects of cyclooxygenase inhibitors on bile-injured and normal equine colon.Campbell NB, Jones SL, Blikslager AT. | Equine Vet J 34:493-498.
  • (2002) Neutrophils increase paracellular permeability of restituted ischemic injured porcine ileum.Gayle J, Jones SL, Argenzio RA, Blikslager AT. | Surgery 132:461-470.
  • (2002) The effects of cAMP modulation upon the adhesion and respiratory burst activity of immune complex-stimulated equine neutrophils.Chilcoat CD, Rowlingson KA, Jones SL. | Vet Immunol Immunopathol 88:65-77.
  • (2002) Protein kinase A regulates beta-2 integrin avidity in neutrophils.Jones SL. | J Leukoc Biol 71:1042-1048.
  • (2001) Signaling mechanism for equine neutrophil activation by immune complexes.Jones SL, Sharief Y, Chilcoat CD. | Vet Immunol Immunopath 82:87-100.
  • (1998) A role for the actin-bundling protein L- plastin in the regulation of leukocyte integrin function.Jones SL, Wang J, Turck CW, Brown EJ. | Proc Natl Acad Sci, USA. 95:9331-9336.
  • Expression of cyclooxygenase-1 and -2 in naturally occurring squamous cell carcinomas in horsesElce YA, Orsini JA, Blikslager AT | Am J Vet Res 2007 Jan;68(1):76-80. PubMed PMID: 17199422
  • (2002) Role of the enteric nervous system in the pathophysiology of secretory diarrheaJones SL, Blikslager AT | J Vet Intern Med 16:222-228
  • (2001) Future directions in anti-inflammatory therapyJones SL, Blikslager AT | Vet Clin North Am Equine Pract 17:245-262
  • (2000) Role of neutrophils in intestinal mucosal injuryGayle JM, Blikslager AT, Jones SL | J Am Vet Med Assoc 217:498-500
  • (1994) Bromophenacyl bromide binding to the actin-bundling protein l-plastin inhibits inositol triphosphate-independent increase in Ca2+ in human neutrophilsRosales C, Jones SL, McCourt D, Brown EJ | Proc Natl Acad Sci USA 91:3534-3538
  • (1999) Phagocytosis. In: Paul WE (ed)Jones SL, Lindberg FL, Brown EJ | Fundamental Immunology Raven Press, New York, NY. pp 997-1020
  • Functional cooperation between Fc receptors and complement receptors in phagocytesJones SL, Brown EJ | In: van de Winkel JGJ, Capel PJA (eds) Human IgG Fc Receptors RG Landes Co, Georgetown, Texas, 1996