Users who have been trained for independent operation of the analyzers will be given access to the online calendar system where they can schedule their appointments and will be given 24 hour access to the facility. Please contact core manager to schedule a training and request access to the facility.

First time users must go through a formal instrument training provided by the flow core. Instrument training by other fellow lab members is not acceptable, and such users will not be granted access to the instruments.

For first time users, it is required to make an appointment with the core manager to discuss your proposed experiment to be sure the core facility has the capabilities to support your experimental needs.

All appointments require 24 hour notice for cancellations. If you do not notify the core manager in writing, you will be charged.

Billing will be based on either the scheduled time or the actual instrument usage time, whichever is greater.

Users must schedule cell sorting and other facility-assisted appointments directly with the core manager, Lunden Simpson via email, as far in advance as possible.

The Flow Cytometry facility is a BSL-1 facility. Human (this includes established human cell lines as well as primary cells) and non human primate samples may be run as long as they are fixed on our analyzers. Our cell sorter is considered BSL-2, a Class-II Baker Biosafety hood houses our Aurora CS, which allows the sorting of unfixed cells. If you have questions about this, please contact the core manager or EHS.

Users of the facility will automatically be added to a flow cytometry listserv that will keep users up to date with the state of the facility.

• Please remember to acknowledge NCSU Flow Cytometry and Cell Sorting facility in all your publications resulting from the use of instrumentation and services at NCSU flow core. Where appropriate, please acknowledge individual members of the Flow Cytometry Core. Following text may be used to acknowledge facility use:

“Flow Cytometry experiments were performed in the Flow Cytometry and Cell Sorting facility at North Carolina State University- College of Veterinary Medicine.”

• Introduction to Flow Cytometry (Video Tutorial)
• Introduction to Fluorescence (Video Tutorial)
• Introduction to Flow Cytometry Data Analysis (Video Tutorial)
• Doublet Discrimination Tutorial – Video tutorial that covers different strategies to exclude doublets
• Cyto University: Free to ISAC members

• The Flow Cytometry Network: A network to connect with Cytometry community
• BD Biosciences Flow Cytometry Tools
• Purdue University Cytometry Laboratories
• International Society for Advancement of Cytometry (ISAC)
• Biolegend Flow Cytometry Tools
• Optimized Multicolor Immunofluorescence Panels (OMIPs)
• Minimum Information about a Flow Cytometry Experiment

Flow Cytometry Protocols from Biorad
Tips and Tricks for Designing Flow Cytometry Panels

Fundamentals of Flow Cytometry (Video Tutorial)
Introduction of FACS DIVA Software (Video Tutorial)