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Poultry Tumor Diseases 

Overview

The Poultry Tumor Diseases Laboratory (PTDL) provides tumor disease and diagnostic expertise to a variety of internal and external funded avian disease research projects, especially involving Marek’s disease. Some fee for service diagnostic work is also performed with prior approval.

Submitting Samples 

Please follow the guidelines below when submitting samples.

TestsDescriptionRequired Sample
Marek's disease diagnosisReal time PCRTumor frozen (shipped in dry ice) or Tumor imprints collected in FTA cards (shipped at room temperature)
Monitoring Marek's disease vaccines serotype 2Real time PCRFeathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature)
Monitoring Marek's disease vaccines serotype 3Real time PCRFeathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature)
Monitoring Marek's disease vaccines serotype 1Real time PCRFeathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature)
Marek's disease diagnosis and monitoring one vaccine typeReal time PCRTumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature)
Marek's disease diagnosis and monitoring of two vaccines typesReal time PCRTumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature)
Marek's disease diagnosis and monitoring of three vaccines typesReal time PCRTumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature)
Marek's disease Gene expressionReal time PCRContact laboratory for further specifications
Avian tumor histopathologyH&E readingTissues collected in formalin 10%
Marek's disease virus isolationPlaque assayLive animals or Frozen buffy coats (contact laboratory for instructions)
Marek's disease vaccine titration (monovalent)Plaque assayThree vaccine vials in liquid shipped in nitrogen or dry ice
Marek's disease vaccine titration (bivalent)Plaque assayThree vaccine vials in liquid shipped in nitrogen or dry ice
Marek's disease vaccine titration(trivalent)Plaque assayThree vaccine vials in liquid shipped in nitrogen or dry ice
Differential diagnosis avian tumorsReal time PCR: Marek's disease
Virus isolation: REV, ALV PCR: REV, ALV
Tumors frozen (shipped in dry ice) and frozen plasma
Immunohistochemistry (MD, cell phenotype)ImmunohistochemistryTissues frozen in OCT and shipped in dry ice (contact laboratory for instructions)
PathotypingGold standard pathotyping assayLive animals
or
Frozen buffy coats (contact laboratory for instructions)
or
Marek’s disease virus in cell culture
or
Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice
NeuropathotypingNeuro signsLive animals
or
Frozen buffy coats (contact laboratory for instructions)
or
Marek’s disease virus in cell culture
or
Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice
Peripheral neuropathy diagnosisReal time PCR HistopathologyFrozen nerves shipped in dry ice and nerves fixed in formalin 10%

Required Paperwork

Sample Submission Form – Poultry Tumor Diseases Laboratory

Click to download form – fee schedule is included – to complete and submit with samples.

Ship samples to:

Attn. Lab Supervisor
North Carolina State University
College of Veterinary Medicine
1060 William Moore Drive
Raleigh, NC 27607 USA

Research Team