AffiliationsSociety for Neuroscience
American Society for Cell Biology
International Society for Stem Cell Research
Biological Barriers, Computational Biology and Bioinformatics, Genetics, Neurobiology, Regenerative MedicineProjects in our laboratory are focused on developmental neurobiology:
1. Development and aging of the adult stem cells and their ependymal niche in the forebrain. We use mouse genetics in combination with molecular, biochemical, and cell biological approaches to address fundamental questions regarding the functional significance of ependymal cells during development and aging.
2. Role of cell cycle regulators in symmetric and asymmetric divisions of neural stem cells in the developing and postnatal brain. We use mouse genetics, biochemical assays, and state-of-the-art imaging tools to understand mechanisms that regulate the decision of neural stem cells to divide symmetrically or asymmetrical in the embryonic and postnatal stem cell niches.
- (2016) TAK1 determines susceptibility to endoplasmic reticulum stress and leptin resistance in the hypothalamus.Sai K, Morioka S, Takaesu G, Muthusamy N, Ghashghaei HT, Hanafusa H, Matsumoto K, Ninomiya-Tsuji J. | J Cell Sci. 2016 May 1;129(9):1855-65. doi: 10.1242/jcs.180505. Epub 2016 Mar 16.
- (2016) TransOmic analysis of forebrain sections in Sp2 conditional knockout embryonic mice using IR-MALDESI imaging of lipids and LC-MS/MS label-free proteomics.Loziuk P, Meier F, Johnson C, Ghashghaei HT, Muddiman DC. | Anal Bioanal Chem. 2016 May;408(13):3453-74. doi: 10.1007/s00216-016-9421-3. Epub 2016 Mar 4.
- (2016) Neurotypic cell attachment and growth on III-nitride lateral polarity structuresBain LE, Kirste R, Johnson CA, Ghashghaei HT, Collazo R, Ivanisevic A | Mater Sci Eng C Mater Biol Appl. 58:1194-8
- (2015) Muddiman D.C. Influence of Desorption Conditions on Analyte Sensitivity and Internal Energy in Discrete Tissue or Whole Body Imaging by IR-MALDESIRosen E.P., Bokhart M.T., Ghashghaei H.T. | J Am Soc Mass Spectrom PMID: 25840812 [Epub ahead of print]
- (2015) Unique glycan signatures regulate adeno-associated viral tropism in the developing brainMurlidharan G., Corriher T., Ghashghaei H.T., Asokan A | J Virol pii: JVI.02951-14. [Epub ahead of print]
- (2014) MARCKS-dependent mucin clearance and lipid metabolism in ependymal cells is required for maintenance of forebrain homeostasis during agingMuthusamy, N., L. Sommerville, A. Moeser, D. Stumpo, P. Sannes, K. Adler, P. Blackshear, J. Weimer, H.T. Ghashghaei | Aging Cell Oct; 14(5):764-73.
- (2014) Transplantation of GABAergic Interneurons into the Neonatal Primary Visual Cortex Reduces Absence Seizures in Stargazer MiceHammad M., Schmidt S.L., Zhang X., Bray R., Frohlich F., Ghashghaei H.T. | Cereb Cortex [Epub ahead of print]
- (2014) A Knock-in Foxj1CreERT2::GFP mouse for recombination in epithelial cells with motile ciliaMuthusamy N., Vijayakumar A., Cheng J., Ghashghaei H.T. | Genesis 52(4):350-8.
- (2013) Neural development is dependent on the function of specificity protein 2 in cell cycle progressionLiang H., Xiao G., Yin H., Hippenmeyer S., Horowitz J.M., Ghashghaei H.T. | Development 140(3):552-61
- (2013) Identification of neuronal loci involved with displays of affective aggression in NC900 miceNehrenberg D.L., Sheikh A., Ghashghaei H.T. | Brain Struct. Funct. 218(4):1033-49
- (2012) A Nestin-cre transgenic mouse isinsufficient for recombination in early embryonic neural progenitors.Liang H., Hippenmeyer S., Ghashghaei H.T. | Biol Open. 1(12):1200-3.
- (2011) Specification of a Foxj1-dependent lineage in the forebrain is required for embryonic-to-postnatal transition of neurogenesis in the olfactory bulbJacquet B.V., Muthusamy N., Sommerville L.J., Xiao G., Liang H., Zhang Y., Holtzman M.J., Ghashghaei H.T. | Journal of Neuroscience 31(25):9368-82
- (2010) An organotypic slice assay for high-resolution time-lapse imaging of neuronal migration in the postnatal brainJacquet B.V., Ruckart P., Ghashghaei H.T. | J Vis Exp (46). pii: 2486. doi: 10.3791/2486
- (2009) FoxJ1-dependent gene expression is required for differentiation of radial glia into ependymal cells and a subset of astrocytes in the postnatal brainJacquet B.V.,Salinas-Mondragon R., Liang H., Therit B., Buie J.D., Dykstra M., Campbell K., Ostrowski L.E., Brody S.L.,and Ghashghaei H.T. | Development 136, 4021-4031.
- (2009) Analysis of neuronal proliferation, migration and differentiation in the postnatal brain using equine infectious anemia virus-based lentiviral vectorsJacquet B.V., Patel M., Iyengar M., Liang H., Therit B., Salinas-Mondragon R., Lai C., Olsen J.C., Anton E.S., Ghashghaei H.T. | Gene Therapy 16(8):1021-33
- (2007) Radial glial dependent and independent dynamics of interneuronal migration in the developing cerebral cortexYokota, Y., Ghashghaei, H.T., Han, C., Watson, H., Campbell, K.J., Anton, E.S. | PLoS ONE 2(8): e794
- (2007) Neuronal migration in the adult brain: are we there yet?Ghashghaei H.T., Lai C., and Anton E.S. | Nature Reviews Neuroscience 8(2):141-151
- (2007) Reinduction of ErbB2 in astrocytes promotes radial glial progenitor identity in adult cerebral cortexGhashghaei H.T., Weimer J.M., Schmid R.S., Yokota Y., McCarthy K.D., Popko B., Anton E.S. | Genes Dev 21(24): 3258-71
- (2006) The role of neuregulin-ErbB4 interactions on the proliferation and organization of cells in the subventricular zoneGhashghaei H.T., Weber J., Pevny L., Schmid R., Schwab M.H., Lloyd K.C., Eisenstat D.D., Lai C., Anton E.S. | Proceeding of the National Academy of Sciences 103(6): 1930-1935
- (2004) Receptor tyrosine kinase ErbB4 modulates neuroblast migration and placement in the adult forebrainAnton E.S.*, Ghashghaei H.T.*, Weber J.L., McCann C., Fischer T.M., Cheung I.D., Gassmann M., Messing A., Klein R., Schwab M.H., Lloyd K.C.K., Lai C. | Nature Neuroscience 7(12): 1319-1328. *Authors contributed equally
Neural Transplant Reduces Absence Epilespsy Seizures in Mice
Stargazer mouse looks upward at transplanted GABAergic neuron (green) in its primary visual cortex. Art by Alice Harvey. New research from North Carolina State University pinpoints the areas of the cerebral cortex that are affected in mice with absence epilepsy and shows that transplanting embryonic neural cells into these areas can alleviate symptoms of the
Researchers Find Lack of Protein Sp2 Disrupts Neuron Creation in Brain
A protein known as Sp2 is key to the proper creation of neurons from stem cells, according to researchers at North Carolina State University. Understanding how this protein works could enable scientists to “program” stem cells for regeneration, which has implications for neural therapies. Troy Ghashghaei and Jon Horowitz, both faculty in the College of
CVM Neurobiologist Maps Areas of the Brain Associated with Affective Aggression in Mice
A North Carolina State University College of Veterinary Medicine researcher has created a roadmap to areas of the brain associated with affective aggression in mice. This roadmap may be the first step toward finding therapies for humans suffering from affective aggression disorders that lead to impulsive violent acts. Affective aggression differs from defensive aggression or
Research Profile: Dr. Ghashghaei
Dr. Troy Ghashghaei, assistant professor of neurobiology, is a neuroscientist in the Department of Molecular and Biomedical Sciences at NC State University’s College of Veterinary Medicine and a member of the NC State Center for Comparative Medicine and Translational Research. Dr. Ghashghaei’s team focuses on discovering genes connected to new brain cell production, and identifying
CCMTR Researchers Identify Genetic Conductor Involved with New Brain Cell Production in Adults
A team of researchers in North Carolina State University’s Center for Comparative Medicine and Translational Research has discovered more about how a gene connected to the production of new brain cells in adults does its job. The findings could pave the way to new therapies for brain injury or disease. Most areas of the brain
Journal of Visualized Experiments Profiles CVM Research Technique
The Journal of Visualized Experiments (JOVE) published a procedure article by Dr. Troy Ghashghaei and research associate Benoit Jacquet titled,” An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain.” Read the abstract, access a PDF of the article, and view the video here. JOVE combines written protocols with videos