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Jorge Piedrahita, PhD

Randall B. Terry, Jr. Distinguished Professor of Translational Medicine & Director, Comparative Medicine Institute

Contact:

japiedra@ncsu.edu
Office: 919.515.7407
Lab: Clinical Studies Core
Lab Phone: 919.515.8380
Lab Fax: 919.513.6680
Center: Comparative Medicine Institute

Dr. Piedrahita obtained his M.Sc. in Reproductive Physiology and his Ph.D. in Cell and Developmental Biology from the University of California. He then moved to the University of North Carolina, Chapel Hill where he completed his Postdoctorate training in stem cells and homologous recombination. He started his academic career at Texas A&M University where he rose from Assistant Professor to Full Professor. He has been at NC State since 2002. He is presently a Professor in the department of Molecular Biomedical Sciences and the Director of the Comparative Medicine Institute (CMI), a university-wide institute.
Affiliations
International Embryo Technologies Society, Society for the Study of Reproduction
Regenerative Medicine
Dr. Piedrahita's laboratory is primarily interested in the behavior of stem and progenitor cells in vitro and in vivo, and the development of large animal models of use in human and veterinary regenerative medicine. Towards this end, his research combines techniques in functional genomics, stem cells, cell biology, embryo manipulation, and molecular biology.
  • (2015) Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.Hensley MT, de Andrade J, Keene B, Meurs K, Tang J, Wang Z, Caranasos TG, Piedrahita J, Li TS, Cheng K. | J Cell Mol Med. 2015 Apr 9. doi: 10.1111/jcmm.12585. [Epub ahead of print]
  • (2015) Generation of Induced Pluripotent Stem Cells (iPSCs) from Adult Canine Fibroblasts.Koh S, Piedrahita JA. | Methods Mol Biol. 2015;1330:69-78. doi: 10.1007/978-1-4939-2848-4_7.
  • (2015) Intravenous Cardiac Stem Cell-Derived Exosomes Ameliorate Cardiac Dysfunction in Doxorubicin Induced Dilated Cardiomyopathy.Vandergriff AC, de Andrade JB, Tang J, Hensley MT, Piedrahita JA, Caranasos TG, Cheng K. | Stem Cells Int. 2015;2015:960926. doi: 10.1155/2015/960926. Epub 2015 Aug 17.
  • (2014) Stem cells and regenerative medicine in domestic and companion animals: a multispecies perspective.Gonçalves NN, Ambrósio CE, Piedrahita JA. | Reprod Domest Anim. 2014 Oct;49 Suppl 4:2-10. doi: 10.1111/rda.12392. Review.
  • (2013) Alternating current electric fields of varying frequencies: effects on proliferation and differentiation of porcine neural progenitor cells.Lim JH, McCullen SD, Piedrahita JA, Loboa EG, Olby NJ. | Cell Reprogram. 2013 Oct;15(5):405-12. doi: 10.1089/cell.2013.0001. Epub 2013 Aug 20.
  • (2013) Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration.Gonzalez LM, Williamson I, Piedrahita JA, Blikslager AT, Magness ST. | PLoS One 2013 Jun 28;8(6):e66465. doi: 10.1371/journal.pone.0066465. Print 2013.
  • (2013) Differentially expressed microRNAs and affected biological pathways revealed by modulated modularity clustering (MMC) analysis of human preeclamptic and IUGR placentas.Guo L, Tsai SQ, Hardison NE, James AH, Motsinger-Reif AA, Thames B, Stone EA, Deng C, Piedrahita JA. | Placenta. 2013 Jul;34(7):599-605. doi: 10.1016/j.placenta.2013.04.007. Epub 2013 Apr 30.
  • (2013) Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins.Bischoff SR, Tsai SQ, Hardison NE, Motsinger-Reif AA, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA. | PloS One 8:e55345
  • (2013) Growth requirements and chromosomal instability of induced pluripotent stem cells (iPSC) generated from adult canine fibroblasts.Koh S, Thomas R, Tsai S, Bischoff S, Lim JH, Breen M, Olby NJ, Piedrahita J. | Stem Cells Dev. 22:951-963.
  • (2012) Isolation and characterization of neural progenitor cells from the adult canine brain.Lim JH, Koh S, Olby NJ, Piedrahita JA, Mariani CL. | Am J Vet Res. 2012 Dec;73(12):1963-8. doi: 10.2460/ajvr.73.12.1963.
  • (2011) Transcriptional profiling of human placentas from pregnancies complicated by preeclampsia reveals disregulation of sialic acid acetylesterase and immune signaling pathways.Tsai S, Hardison NE, James AH, Motsinger-Reif AA, Bischoff SR, Thames BH, and Piedrahita JA. | Placenta 32:175-182.
  • (2010) Development of a model of sacrocaudal spinal cord injury in cloned Yucatan minipigs for cellular transplantation research. Lim JH, Piedrahita JA, Jackson L, Ghashghaei T, Olby NJ. | Cellular Reprogramming 12:689-697.
  • (2010) Generation and characterization of neurospheres from canine adipose tissue-derived stromal cells.Lim JH, Boozer L, Mariani CL, Piedrahita JA, Olby NJ. | Cellular Reprogramming 12:417-425.
  • (2010) Varying phenotypes in swine versus murine transgenic models consitutively expressing the same human sonic hedgehog transcriptional activators, K5-hGLI2 delat N. McCall-Martin A, Chen X, Linder KE, Estrada JL, Piedrahita JA. | Transgenic Res. 2010 Oct;19(5):869-87. doi: 10.1007/s11248-010-9362-0. Epub 2010 Jan 23.
  • (2009) Characterization of conserved and non-conserved imprinted genes in swine.Bischoff SR, Tsai S, Hardison N, Motsinger-Reif A, Freking BA, Nonneman D, Rohrer G, Piedrahita JA. | Biol. Reprod. 81:906-920.
  • (2008) Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ)Zaunbrecher G, Mir B, Dunne PW, Breen M, Piedrahita JA | Animal Biotechnology 19:6-21
  • (2008) A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: effects of the squeezing and the aspiration methodsLee E, Estrada JL, Piedrahita JA | Animal Biotechnology 19:71-79
  • (2008) Successful cloning of the Yucatan minipig using commercial/occidental breeds as oocyte donors and embryo recipientsEstrada JL, Collins B, York A, Bischoff SR, Sommer J, Tsai S, Petters RM, Piedrahita JA | Cloning and Stem Cells 10:287-296
  • (2008) Identification of SNPs and INDELS in swine using short oligonucleotide microarraysBischoff SR, Tsai, S, Hardison NE, York AM, Freking BA, Nonneman D, Rohrer G, Piedrahita JA | BMC Genomics 9:252-263
  • (2008) Successful cloning of the Yucatan minipig using commercial/occidental breeds as oocyte donors and embryo recipientsEstrada JL, Collins B, York A, Bischoff SR, Sommer J, Tsai S, Petters RM, Piedrahita JA | Cloning and Stem Cells 10:287-296
  • (2008) Identification of SNPs and INDELS in swine using short oligonucleotide microarraysBischoff SR, Tsai, S, Hardison NE, York AM, Freking BA, Nonneman D, Rohrer G, Piedrahita JA | BMC Genomics 9:252-263
  • (2008) Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ)Zaunbrecher G, Mir B, Dunne PW, Breen M, Piedrahita JA | Animal Biotechnology 19:6-21
  • (2008) A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: effects of the squeezing and the aspiration methodsLee E, Estrada JL, Piedrahita JA | Animal Biotechnology 19:71-79
  • (2007) Swine generated by somatic cell nuclear transfer have increased incidence of intra uterine growth restriction (IUGR)Estrada J, Sommer J, Collins B, Mir B, Martin A, York A, Petters RM, and Piedrahita JA | Cloning and Stem Cells 9:229-236
  • (2007) Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR)Estrada J, Sommer J, Collins B, Mir B, Martin A, York A, Petters RM, Piedrahita JA | Cloning Stem Cells 2007 Summer;9(2):229-36
  • (2007) Swine generated by somatic cell nuclear transfer have increased incidence of intra uterine growth restriction (IUGR)Estrada J, Sommer J, Collins B, Mir B, Martin A, York A, Petters RM, and Piedrahita JA | Cloning and Stem Cells 9:229-236
  • (2007) Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR)Estrada J, Sommer J, Collins B, Mir B, Martin A, York A, Petters RM, Piedrahita JA | Cloning Stem Cells 2007 Summer;9(2):229-36
  • (2006) Annotation of the Affymetrix porcine genome microarrayTsai S, Cassady JP, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA | Anim Genet 37:423-4
  • (2006) Dermatological effects of chronic exposure to 7,12-dimethylbenz[Ajanthracene(DMBA) or N-methyl-N-nitrosoguanidine (MNNG) in swineMonteiro-Riviere N, Inman A, Hedgpeth V, Mosteller B, Piedrahita J | Cutan Ocul Toxicol 25:103-19
  • (2006) Errors in development of fetuses and placentas from in vitro-produced bovine embryosFarin PW, Piedrahita JA, Farin CE | Theriogenology 65:178-91
  • (2006) Dermatological effects of chronic exposure to 7,12-dimethylbenz[a]anthracene (DMBA) of n-methyl-n-nitrosoguanidine (MNNG) in swineMonteiro_Riviere N, Hedgepeth N, Inman A, Mosteller B, and Piedrahita JA | J Cutanoeus Ocular Tox 25:103-119
  • (2006) Annotation of Affymetrix Porcine genome Microarray ChipTsai S, Cassady J, Freking D, Nonneman G. Rohrer G, Piedrahita JA | Animal Genetics 37:423-424
  • (2006) Detection of transcriptional difference of porcine imprinted genes using different microarray platformsTsai S, Mir B, Martin AC, Estrada JL, Bischoff SR, Hsieh WP, Cassady JP, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA | BMC Genomics 7:328-336
  • (2006) Annotation of the Affymetrix porcine genome microarrayTsai S, Cassady JP, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA | Anim Genet 37:423-4
  • (2006) Dermatological effects of chronic exposure to 7,12-dimethylbenz[Ajanthracene(DMBA) or N-methyl-N-nitrosoguanidine (MNNG) in swineMonteiro-Riviere N, Inman A, Hedgpeth V, Mosteller B, Piedrahita J | Cutan Ocul Toxicol 25:103-19
  • (2006) Errors in development of fetuses and placentas from in vitro-produced bovine embryosFarin PW, Piedrahita JA, Farin CE | Theriogenology 65:178-91
  • (2006) Dermatological effects of chronic exposure to 7,12-dimethylbenz[a]anthracene (DMBA) of n-methyl-n-nitrosoguanidine (MNNG) in swineMonteiro_Riviere N, Hedgepeth N, Inman A, Mosteller B, and Piedrahita JA | J Cutanoeus Ocular Tox 25:103-119
  • (2006) Annotation of Affymetrix Porcine genome Microarray ChipTsai S, Cassady J, Freking D, Nonneman G. Rohrer G, Piedrahita JA | Animal Genetics 37:423-424
  • (2006) Detection of transcriptional difference of porcine imprinted genes using different microarray platformsTsai S, Mir B, Martin AC, Estrada JL, Bischoff SR, Hsieh WP, Cassady JP, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA | BMC Genomics 7:328-336
  • (2005) Progeny of somatic cell nuclear transfer (SCNT) clones of phenotypically similar to non-cloned pigsMir B, Zaunbrecher G, Archer GS, Friend T, and Piedrahita JA | Cloning and Stem Cells 7:119-125
  • (2005) Progeny of somatic cell nuclear transfer (SCNT) clones of phenotypically similar to non-cloned pigsMir B, Zaunbrecher G, Archer GS, Friend T, and Piedrahita JA | Cloning and Stem Cells 7:119-125
  • (2004) Conservation of genomic imprinting at the XIST, IGF2, and GTL2 loci in the bovineDindot S, Kent KC, Evers B, Loskutoff N, Womack J and Piedrahita JA | Mammalian Genome 15:828-833
  • (2004) Somatic cell cloning: The ultimate form of nuclear reprogramming?Piedrahita JA, Mir B, Dindot S, and Walker S | J Amer. Soc. Nephrol. 15:1140-1144
  • (2004) Development of fetuses from in vitro produced and cloned bovine embryosFarin C, Farin PW, and Piedrahita JA | J. Animal Sc. 82:53-62
  • (2004) Epigenetic and genomic imprinting analysis in nuclear transfer derived Bos gaurus/Bos taurus hybrid fetusesDindot S, Farin C, Farin P, Miles J, Walker S, Long C, and Piedrahita JA | Biol. Reprod. 71:470-478
  • (2004) Nuclear localization signal and cell synchrony enhances homologous recombination in fetal fibroblastsMir B and Piedrahita JA | Nucleic Acid Res. 11:e25-e28
  • (2004) Cloning and Transgenesis in Mammals; Implications for XenotransplantationPiedrahita JA and Mir B | Am. J. Transpl. 6:43-50
  • (2004) Use of artificial androgen receptor coactivators to alter myoblast proliferationBenjamin CL, Jenster G, and Piedrahita JA | J. Steriod Bioch. Mol. Biol. 91:111-119
  • (2004) Somatic cell cloning: The ultimate form of nuclear reprogramming?Piedrahita JA, Mir B, Dindot S, and Walker S | J Amer. Soc. Nephrol. 15:1140-1144
  • (2004) Development of fetuses from in vitro produced and cloned bovine embryosFarin C, Farin PW, and Piedrahita JA | J. Animal Sc. 82:53-62
  • (2004) Epigenetic and genomic imprinting analysis in nuclear transfer derived Bos gaurus/Bos taurus hybrid fetusesDindot S, Farin C, Farin P, Miles J, Walker S, Long C, and Piedrahita JA | Biol. Reprod. 71:470-478
  • (2004) Nuclear localization signal and cell synchrony enhances homologous recombination in fetal fibroblastsMir B and Piedrahita JA | Nucleic Acid Res. 11:e25-e28
  • (2004) Cloning and Transgenesis in Mammals; Implications for XenotransplantationPiedrahita JA and Mir B | Am. J. Transpl. 6:43-50
  • (2004) Use of artificial androgen receptor coactivators to alter myoblast proliferationBenjamin CL, Jenster G, and Piedrahita JA | J. Steriod Bioch. Mol. Biol. 91:111-119
  • (2004) Conservation of genomic imprinting at the XIST, IGF2, and GTL2 loci in the bovineDindot S, Kent KC, Evers B, Loskutoff N, Womack J and Piedrahita JA | Mammalian Genome 15:828-833
  • (2003) Behavioral Variation in Cloned PigsArcher G, Friend, T, Piedrahita JA, Neville C, Walker SC | Applied Animal Behavior 2012:1-11
  • (2003) Kidney-specific activity of bovine uromodulin promoter in transgenic miceKim H-T, Song I-Y, Piedrahita JA | Transgenic Research 12:191-201
  • (2003) UP1 Extends Life of Primary Porcine FetalFibroblasts in CultureMir B, Tanner N, Chowdhary BP, Piedrahita JA | Cloning and Stem Cells 5: 143-148
  • (2003) Hierarchical Phenotypic and Epigenetic Variation in Cloned SwineArcher GS, Dindot S, Friend TH, Walker S, Zaunbrecher G, Lawhorn B, Piedrahita JA | Biol. Reprod. 69:430-436
  • (2003) Mutational analysis of loxP sites for efficient cre-mediated insertion into genomic DNAThomson J, Rucker E, and Piedrahita JA | Genesis 36:162-167
  • (2003) Behavioral Variation in Cloned PigsArcher G, Friend, T, Piedrahita JA, Neville C, Walker SC | Applied Animal Behavior 2012:1-11
  • (2003) Kidney-specific activity of bovine uromodulin promoter in transgenic miceKim H-T, Song I-Y, Piedrahita JA | Transgenic Research 12:191-201
  • (2003) UP1 Extends Life of Primary Porcine FetalFibroblasts in CultureMir B, Tanner N, Chowdhary BP, Piedrahita JA | Cloning and Stem Cells 5: 143-148
  • (2003) Hierarchical Phenotypic and Epigenetic Variation in Cloned SwineArcher GS, Dindot S, Friend TH, Walker S, Zaunbrecher G, Lawhorn B, Piedrahita JA | Biol. Reprod. 69:430-436
  • (2003) Mutational analysis of loxP sites for efficient cre-mediated insertion into genomic DNAThomson J, Rucker E, and Piedrahita JA | Genesis 36:162-167
  • (2002) Effects of Follicular Size of Cytoplast Donor on the Efficiency of Cloning in CattlePiedrahita JA , Wells DN, Miller AL, Oliver JE , Berg MC , Peterson AJ, Tervit RH | Mol. Reprod. Dev. 61:317-326
  • (2002) Inhibition of apoptosis in serum starved porcine embryonic fibroblastsLee CK, Piedrahita JA | Mol. Reprod. Devel. 62:106-112
  • (2002) A Highly Efficient Method for Porcine Cloning by Nuclear Transfer Using In Vitro-Matured OocytesWalker SC, Shin TY, Zaunbrecher GM, Romano JE, Johnson, GA, Bazer, FW and Piedrahita JA | Cloning Stem Cells 4:105-112
  • (2002) Effects of Follicular Size of Cytoplast Donor on the Efficiency of Cloning in CattlePiedrahita JA , Wells DN, Miller AL, Oliver JE , Berg MC , Peterson AJ, Tervit RH | Mol. Reprod. Dev. 61:317-326
  • (2002) Inhibition of apoptosis in serum starved porcine embryonic fibroblastsLee CK, Piedrahita JA | Mol. Reprod. Devel. 62:106-112
  • (2002) A Highly Efficient Method for Porcine Cloning by Nuclear Transfer Using In Vitro-Matured OocytesWalker SC, Shin TY, Zaunbrecher GM, Romano JE, Johnson, GA, Bazer, FW and Piedrahita JA | Cloning Stem Cells 4:105-112
  • (2000) Effects of protease inhibitors and antioxidants on in vitro survival of porcine primordial germ cellsLee C-K, Weaks R, Johnson G, Bazer F , and Piedrahita, JA | Biol. Reprod. 63:887-897
  • (2000) Effects of growth factors and feeder cells on porcine primordial germ cells in vitroLee C-K, and Piedrahita JA | Cloning 2:197-206
  • (2000) Three little pigs worth the huff and puff?Westhusin M, and Piedrahita JA | Nature Biotechnol 18:1144-1145
  • (2000) Gene targeting in domestic species: A new beginningPiedrahita JA | Transgenic Res 9:261-262
  • (2000) Effects of protease inhibitors and antioxidants on in vitro survival of porcine primordial germ cellsLee C-K, Weaks R, Johnson G, Bazer F , and Piedrahita, JA | Biol. Reprod. 63:887-897
  • (2000) Effects of growth factors and feeder cells on porcine primordial germ cells in vitroLee C-K, and Piedrahita JA | Cloning 2:197-206
  • (2000) Three little pigs worth the huff and puff?Westhusin M, and Piedrahita JA | Nature Biotechnol 18:1144-1145
  • (2000) Gene targeting in domestic species: A new beginningPiedrahita JA | Transgenic Res 9:261-262
  • (1999) Inactivation of the folate binding protein genes disrupts neural tube closurePiedrahita JA , Oetama B, Bennett G, Waes JV, Lacey SW, Kamen B, Richardson J, Lark R, and Finnell R | Nature Genetics 23:228-232
  • (1999) Regulated Expression of MHC class I molecules in the porcine embryoRamsoondar JJ, Christopherson RJ, Guilbert LJ, Dixon WT, Ghahary A, Ellis S, Wegmann TG, Piedrahita JA | Biol. Reprod. 60:387-397
  • (1999) Inactivation of the folate binding protein genes disrupts neural tube closurePiedrahita JA , Oetama B, Bennett G, Waes JV, Lacey SW, Kamen B, Richardson J, Lark R, and Finnell R | Nature Genetics 23:228-232
  • (1999) Regulated Expression of MHC class I molecules in the porcine embryoRamsoondar JJ, Christopherson RJ, Guilbert LJ, Dixon WT, Ghahary A, Ellis S, Wegmann TG, Piedrahita JA | Biol. Reprod. 60:387-397
  • (1998) Factors affecting the efficiency of introducing small mutation in specific genes by homologous recombination; Cre-loxP versus the tag-and-exchangeVazquez JC, Nogues C, Rucker E, and Piedrahita JA | Transgenic Research 7 181-193
  • (1998) Generation of transgenic porcine chimeras using primordial germ cell (PGC)-derived coloniesPiedrahita JA , Moore K, Oetama B, Lee C-K, Scales N, Ramsoondar J, Bazer F, and Ott T | Biol. Reprod. 58, 1321-1329
  • (1998) Generation of transgenic porcine chimeras using primordial germ cell (PGC)-derived coloniesPiedrahita JA , Moore K, Oetama B, Lee C-K, Scales N, Ramsoondar J, Bazer F, and Ott T | Biol. Reprod. 58, 1321-1329
  • (1998) Factors affecting the efficiency of introducing small mutation in specific genes by homologous recombination; Cre-loxP versus the tag-and-exchangeVazquez JC, Nogues C, Rucker E, and Piedrahita JA | Transgenic Research 7 181-193
  • (1997) Cre-mediated site-directed insertions at the mouse whey acidic protein (mWAP) locusRucker E, and Piedrahita JA | Mol. Repr. Devel. 48: 324-331
  • (1997) Cre-mediated site-directed insertions at the mouse whey acidic protein (mWAP) locusRucker E, and Piedrahita JA | Mol. Repr. Devel. 48: 324-331
  • (1992) Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in mouse embryonic stem cellsPiedrahita JA , Zhang SH, Hagaman JR, Clark PM, and Maeda N | Proc. Natl. Acad. Sci. USA 89:4471-4475
  • (1992) Production of chimeric hamsters by aggregation of eight-cell embryosPiedrahita JA , Gillespie L, and Maeda N. | Biology of Reproduction 47:347-354
  • (1992) Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein EZhang SH, Reddick RL, Piedrahita JA, and Maeda N | Science 258:468-471
  • (1992) Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in mouse embryonic stem cellsPiedrahita JA , Zhang SH, Hagaman JR, Clark PM, and Maeda N | Proc. Natl. Acad. Sci. USA 89:4471-4475
  • (1992) Production of chimeric hamsters by aggregation of eight-cell embryosPiedrahita JA , Gillespie L, and Maeda N. | Biology of Reproduction 47:347-354
  • (1992) Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein EZhang SH, Reddick RL, Piedrahita JA, and Maeda N | Science 258:468-471