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College of Veterinary Medicine

Flow Cytometry and Cell Sorting

If you are looking for the Clinical Pathology & Immunology diagnostic testing lab service please click here.

Instrumentation

High Speed Cell Sorter

Beckman Coulter MoFlo XDP

Beckman Coulter MoFlo XDP
The Beckman Coulter MoFlo XDP provides NCSU and to those outside of the university community with the flexibility to perform a wide range of cell sorting experiments. Our XDP is configured with a 200mW 488nm laser, a 35mW 640nm laser, and two OBIS “plug and play” lasers; a 100mW 405nm laser and a 50mW 561nm laser. The sorter is capable of analyzing up to 10 colors plus forward and side scatter. In terms of sorting capability, the XDP can sort for up to four different cell populations simultaneously, and is capable of purifying populations that are less than 1% of the total input sample. We have the ability to sort a wide variety of cell types due to the variable sizes of our interchangeable nozzle tips. Please contact us to discuss about your experiment and schedule a sorting.

Analyzers

Becton Dickinson LSRII

Becton Dickinson LSRII: Has three lasers, 405 nm (Violet), 488 nm (Blue) and 635 nm (Red). Can analyze up to nine colors and utilizes BD Diva software for acquisition. Click here to access the LSRII configuration for experiment planning.

Beckman Coulter MoFlo XDP


Beckman Coulter CytoFLEX: Has 405 nm (Violet), 488 nm (Blue) and 633 nm (Red) lasers. Supports up to nine parameters and has a vSSC parameter for detection of nanoparticles at higher resolution. The CytoFlex configuration is customizable for specific experiments. The Cytoflex can perform absolute counts. Click here to access instrument configuration.

Automated Cell Counter

LunaII


LunaII: The LunaII is an automated cell counter that supports brightfield cell counting. It has features to auto-focus, adjust light levels and capture a high-resolution image. You can create custom, cell-specific cell counting protocols or simply use default counting protocol suitable for most cell types.

Scheduling

MoFlo-XDP Cell Sorter: Scheduling for cell sorting is only available though the flow cytometry core manager. Please contact us to discuss about your experimental needs and schedule a sorting.

LSRII and CytoFLEX: Scheduling for the LSRII and CytoFLEX is available through online calendar. Users are given access to the scheduler after completing required introductory and/or instrument training. Please contact the core manager to schedule introductory and analyzer training.

Special Filters

ExcitationFluorescence ChannelsFluorochromes
488nm525/40 BPFitc, alexa Fluor 488, CFSE, Fluo-3
585/42 BPPE, PI
610/20 BPECD, PE-Texas Red, PE-CF594, PI
690/50 BPPC5, PC5.5, PerCP, PerCP-cy5.5, PI
780/60 BPPC7
638nm660/20 BPAPC, Alexa Fluor 647, eFluor 660
712/25 BPAPC-Alexa Fluor 700, Alexa Fluor 700
780/60 BPAPC-Alexa Fluor 750, APC-Cy7, APC-H7, APC-eFluor 780
405nm450/45 BPPacific Blue, V450, eFluor 450, BV421
525/40 BPKrome Orange, AmCyan, V500, BV510
610/20 BPViolet610, BV605, Qdot 605
660/20 BPViolet660, BV650, Qdot 655
780/60 BPViolet780, BV785, Qdot 800

Services

  • Introductory Training
  • Instrument Training
  • Assisted Acquisition/Analysis
  • Cell Sorting
  • Analysis Software
  • Experiment Design/Consultation

Facility Policies

Users who have been trained for independent operation of the analyzers will be given access to the online calendar system where they can schedule their appointments and will be given 24 hour access to the facility. Please contact core manager to schedule a training and request access to the facility.

 First time users must go through a formal instrument training provided by the flow core. Instrument training by other fellow lab members is not acceptable, and such users will not be granted access to the instruments.

For first time users, it is required to make an appointment with the core manager to discuss your proposed experiment to be sure the core facility has the capabilities to support your experimental needs.

All appointments require 24 hour notice for cancellations. If you do not notify the core manager in writing, you will be charged.

Billing will be based on either the scheduled time or the actual instrument usage time, whichever is greater.

Users must schedule cell sorting and other facility-assisted appointments directly with the core manager, Lunden Simpson via email, as far in advance as possible.

The Flow Cytometry facility is a BSL-1 facility. Human (this includes established human cell lines as well as primary cells) and non human primate samples may be run as long as they are fixed. If you have questions about this, please contact the core manager or EHS. Also, we are in the process of upgrading our facility to BSL2/2+. We will post the update here once the facility is upgraded to BSL2/2+.

Users of the facility will automatically be added to a flow cytometry listserv that will keep users up to date with the state of the facility.

  • Please remember to acknowledge NCSU Flow Cytometry and Cell Sorting facility in all your publications resulting from the use of instrumentation and services at NCSU flow core. Where appropriate, please acknowledge individual members of the Flow Cytometry Core. Following text may be used to acknowledge facility use:

“Flow Cytometry experiments were performed in the Flow Cytometry and Cell Sorting facility at North Carolina State University- College of Veterinary Medicine.”

Rates

ServiceNCSU (per hour)External Academic (per hour)External Commercial (per hour)
LSRII unassisted$ 59$ 63$ 73
CytoFLEX unassisted$ 59$ 63$ 73
LSRII assisted (includes staff time)$ 188$ 190$ 200
CytoFLEX assisted (includes staff time)$ 188$ 190$ 200
Analysis Computerfreefreefree
Consultation$ 71$ 76$ 81
Seahorse XF Mini- Customer operated$ 63$ 67$ 77
Nanostring - Customer Operated$ 35$ 40$ 45
Luna Cell CounterContact UsContact UsContact Us
FlowJo LicenseContact UsNot AvailableNot Available

Resources

Contact Us

LS
Lunden Simpson

Laboratory Manager

Location:

Flow Cytometry and Cell Sorting Facility

CVM Main Building, Room# B303

North Carolina State University – College of Veterinary Medicine

Raleigh, NC 27607