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Isabel Gimeno, DVM, PhD, DACPV

Associate Professor, Poultry Health Management

Contact:

isabel_gimeno@ncsu.edu
Office: 919.513.6852
Lab: Poultry Tumor Diseases
Lab Phone: 919.513.6333

Professional Experience
July 2012 to present. Associate Professor. Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University.
February 2006 to July 2012. Assistant Professor. Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University.
February 2002 to January 2006. Postdoctoral position at the USDA-ARS Avian Disease and Oncology Laboratory, East Lansing, MI.
January 2001 to January 2002 . Research Associate at the Animal Health Diagnostic Laboratory, Michigan State University, East Lansing, MI
January 2000 to December 2000. Research Associate at the Research Institute for Animal Health (CISA-INIA), Valdeolmos, Madrid.

Certifications
Diplomate, American College of Poultry Veterinarians

Education/Training
Diplomate, American College of Poultry Veterinarians
PhD, Veterinary Pathology, Universidad Complutense de Madrid, 1999
MS, Poultry Diseases and Production, Universidad Complutense de Madrid, 1996
Licenciado en Veterinaria (DVM equivalent) - Universidad Complutense de Madrid, 1995
Infectious Diseases
Marek's disease (MD) is a lymphoproliferative disease induced by the alpha-herpesvirus Marek's disease virus (MDV). MDV has evolved towards more virulent forms in the recent decades. The efficacy of MDV vaccines has decreased concomitantly with the increase in virulence of field isolates. Of particular importance, the most commonly utilized vaccine in the USA during the past 20 years (bivalent vaccine including serotypes 2 and 3) does not protect chickens against the most recently isolated strains of MDV, the very virulent plus (vv+/). Attenuated serotype 1 vaccines seem to confer better protection against challenge with vv+/strains. Nonetheless, even serotype 1 vaccines have been shown to have reduced efficacy with time in some European and Asian countries. Because of the great economic impact of Marek's disease and the threat of more virulent MDV strains emerging, the search for more efficient vaccines continues. A major limitation in the development of more efficient vaccines, however, is the lack of information on mechanisms of vaccine immunity. One of the goals of my laboratory is to better understand the mechanisms of Marek's disease vaccine immunity to develop better strategies of control.

Diagnosis of tumor poultry diseases is complex. My laboratory has worked for several years to establish new diagnostic criteria and standardize the use of molecular techniques for the diagnosis of tumor viral diseases. Furthermore, we have developed real time PCR assays that allow early diagnosis and monitoring of vaccination in the field.

In addition to the development of lymphomas, Marek's disease virus induced a variety of neurological syndromes. The pathogenesis of MDV in the nervous system is not fully understood but it is very likely that Marek's disease virus elicit an abnormal immune response leading to the development of autoimmunity. One of the objectives of my laboratory is to elucidate the mechanisms by which MDV induce lesions in the brain and in the peripheral nerves as well as to determine the factors (virus, host and environment) that contribute to the development of MDV-induced neurological syndromes.

One of our current interests is the study of the immunosuppression induced by MDV (MDV-IS). My laboratory has developed a model to evaluate MDV-IS under laboratory conditions. Further studies using this model will allow a better understanding of the mechanisms behind MDV-IS as well as they will aid in the development of detection and control methods.


Research Areas:
Avian tumor viruses
Avian herpesviruses
Emerging poultry infectious diseases
Poultry immunology
Poultry pathology

  • (2005). Characterization of a very Virulent Marek's Disease Virus Mutant Expressing the pp38 Protein from the Serotype 1 Vaccine Strain CVI988/Rispens.Lee, L.F., X. Cui, Cui, Z.,  I.M. Gimeno, B. Lupiani, S.M. Reddy. | Virus Genes, 31: 73-80.
  • (2005). Novel methods for the diagnosis of Marek’s disease.Gimeno, I.M., R.L. Witter, A.M. Fadly, R.F. Silva. | Avian Pathology, 34: 332-340.
  • (2005). The pp38 gene of Marek's disease virus (MDV) is necessary for cytolytic infection of B cells and maintenance of the transformed state but not for cytolytic infection of the feather follicle epithelium and horizontal spread of MDV.Gimeno, I.M., R.L. Witter, H.D. Hunt, R.F. Silva. | J. Virol. 79:4545-4549.
  • (2004). Marek’s disease virus encoded Meq gene is involved in transformation of lymphocytes but dispensable for early cytolytic infection.Lupiani, B.M., X. Cui, L.F. Lee, I.M. Gimeno, R.W. Morgan, R. Silva, R.L. Witter, H.J. Kung, S.M Reddy. | Proceedings of the National Academy of Science USA, 101: 11815-11820.
  • (2004). Biocharacteristics shared by highly protective vaccines against Marek's disease.Gimeno, I.M., R.L. Witter, H.D. Hunt, S.M. Reddy, W.M. Reed | Avian Pathology, 33: 59-68.
  • (2002). Rescue of a pathogenic Marek’s disease virus using overlapping cosmid DNAs: pp38 is not essential for oncogenesis.Reddy, A.M., B. Lupiani, I.M. Gimeno, L.F. Lee, R.F. Silva, R.L. Witter | Proceedings of the National Academy of Science USA 99:7054-7059 
  • (2002). Neurological response as an additional criterion for the pathotypic classification of Marek’s disease virus.Gimeno, I.M., R.L. Witter, U. Neumann | Avian Diseases, 46:909-918
  • (2001). Differential attenuation of neurological pathology in Marek´s disease virus: a model for studying pathogenesis of transient paralysis.Gimeno, I.M., R.L. Witter, H.D. Hunt, S.M. Reddy, U. Neumann. | Avian Pathology, 30: 397-409.
  • (2001). Marek´s disease virus down regulates surface expression of MHC (B complex) class I (BF) glycoproteins during active but not latent infection of chicken cells.Hunt, H.D., B. Lupiani, M.M. Miller, I.M. Gimeno, L.F. Lee, M.S. Parcells. | Virology, 282: 198-205.
  • (2000). Development of a quantitative-competitive PCR assay for serotype 1 Marek´s disease virus.Reddy, S.M., R.L. Witter, I.M. Gimeno. | Avian Diseases, 44: 770-775.
  • (1999). Evidence for Marek´s disease in turkeys in Germany: Detection of MDVH serotype 1 using the polymerase chain reaction.Voelckel, K., E. Bertram, I.M. Gimeno, U. Neumann, E.F. Kaleta. | Acta Virologica, 43: 143-147.
  • (1999). Four Distinct Neurological Syndromes in Marek´s Disease: Effect of Viral Strain and Pathotype.Gimeno, I.M., R.L. Witter, L.D. Bacon, W.M. Reed. | Avian Diseases, 43: 721-737. 
  • (1999). An acute form of transient paralysis induced by highly virulent strains of Marek´s disease virus.Witter, R.L., I. M. Gimeno, W.M. Reed. | Avian Diseases, 43: 704-720.
  • (2004) Future strategies for controlling Marek’s disease. In Marek’s disease. Edited by F. Davison and V. Nair, ElsevierGimeno, I.M. | pp186-199, London, UK.
  • (2004). Differential diagnosis of lymphoid and myeloid tumors in the chicken. American Association of Avian PathologistWitter, R.L., I.M. Gimeno, A.M. Fadly. | (slide study set #27)
  • Marek’s disease. (2004)Gimeno, I.M., R.L. Witter, A. Miles. | American Association of Avian Pathologist (slide study set #26)
  • (2005) Classification of Marek’s disease according to pathotype – philosophy and methodology.Witter, R.L., B.W. Calnek, C. Buscaglia, I.M. Gimeno, K.A. Schat. | Avian Pathol., 34:75-90 (invited review)
  • (2008) Marek’s disease vaccines: a solution for today but a worry for tomorrow?.Gimeno, I.M. | Vaccine, 26S:C31-C41
  • Enfermedad de Marek. 2009.Gimeno, I.M. | In Principios Basicos de Patologia Aviar. A.J.P. Ferreira and L. Revolledo (eds). Guanabara Koogan, Brasil pp. 193-228
  • 2010 Tumor diagnosis manual. The differential diagnosis of lymphoid and myeloid tumors in the chicken.Witter, R.L., Gimeno,I.M.,  Pandiri, A.K., Fadly, A.M. | First edition, American Association of Avian Pathologists. Jacksonville, Florida.
  • (2010) Evaluation of factors influencing replication of Marek’s disease vaccines in the chicken lung. I.M. Gimeno, A.L. Cortes. | Avian Pathology, 39:71-79
  • (2009) Validation of Marek’s disease diagnosis and monitoring of Marek’s disease vaccines from samples collected in FTA cards.A.L. Cortes, E. R. Montiel., I.M. Gimeno. | Avian Diseases, 53(4):510-6
  • (2008) Marek’s disease virus infection in the eye: chronological study of the lesions, virus replication, and vaccine-induce protection.Pandiri, A.R., A.L. Cortes, L.F. Lee, I.M. Gimeno. | Avian Diseases, 52:572-80.
  • (2009) Role of infection profile on the pathogenesis of subgroup J avian leukosis virus-induced histiocytic sarcomatosis in meat-type chickens.Pandiri, A.R., I.M. Gimeno, W.M. Reed, S.D. Fitzgerald, A.M. Fadly. | Veterinary Pathology. 46:282-7.
  • (2008) Load of challenge Marek’s disease virus DNA in blood as a criterion for early diagnosis of Marek’s disease tumors.Gimeno, I.M., A.L. Cortes, R.F. Silva. | Avian Diseases, 52: 203-208
  • (2008) Biological function of Marek’s disease virus gene UL41.  Gimeno, I.M., R.F. Silva. | Virus Genes, 36: 499-507.
  • (2008) Distribution of viral antigen gp85 and provirus in various tissues from commercial meat-type and experimental white leghorn line 0 chickens with different subgroup J avian leukosis virus infection profiles.Pandiri, A.K.R., I.M. Gimeno, W.M. Reed, L.F. Lee, R.F. Silva, A.M. Fadly. | Avian Pathology, 37: 7-13
  • (2007). Oncogenic Marek's disease viruses lacking the 132 base pair repeats can still be attenuated by serial in vitro cell culture passages.Silva, R.F., I.M. Gimeno. | Virus genes, 34: 87-90.
  • (2006). Susceptibility of adult chickens, with and without prior vaccination, to challenge with Marek’s disease virus.Witter, R.L., I.M. Gimeno | Avian Diseases, 50: 354-365
  • (2014) Detection and Differentiation of CVI988 (Rispens Vaccine) from Other Serotype 1 Marek’s Disease Viruses.I. M. Gimeno, J. Dunn, A.L. Cortes, A.E. El-Gohary, R.F. Silva | Avian Diseases 58 (in press)
  • (2013) Current status of Marek's disease in the United States and worldwide based on a questionnaire survey.Dunn, J., I.M. Gimeno. | Avian Dis. 57:483-90.
  • Witter (2012) Dynamic equilibrium of Marek's disease genomes during in vitro serial passage.Spatz SJ, J.D.Volkening, I.M. Gimeno,  M. Heidar, R.L. | Virus Genes, 45:526-36.
  • (2012) Optimization of the protocols for double vaccination against Marek’s disease using commercially available vaccines: evaluation of protection, vaccine replication, and activation of T cells.Gimeno, I.M., A.L. Cortes, R.L. Witter, A.R. Pandiri | Avian Diseases, 56: 295-305.  
  • (2012) Reactivation of Subgroup J avian leukosis virus (ALV J) viremia persistence following induction of stress in adult seroconverted meat-type chickens.A.R. Pandiri, I.M. Gimeno, J.M. Mays, W.M. Reed, and A.M. Fadly. | Avian Diseases, in press.
  • (2011) Standardization of a model to study revaccination against Marek’s disease under laboratory conditions.Gimeno, I.M., R.L. Witter, A.L. Cortes, S.M. Reddy, A.R. Pandiri | Avian Pathology, 41:59-68.
  • (2011) Reactivation of Subgroup J avian leukosis virus (ALV J) viremia persistence following induction of stress in adult seroconverted meat-type chickens.A.R. Pandiri, I.M. Gimeno, J.M. Mays, W.M. Reed, and A.M. Fadly. | Avian Diseases,
  • (2011) Replication ability of three highly protective Marek’s disease vaccines: implications in lymphoid organ atrophy and protection.Gimeno, I.M., R.L. Witter, A.L. Cortes, W.M. Reed | Avian Pathology, 40:573-6.
  • (2011) Replication of recombinant herpesvirus of turkey expressing genes of infectious laryngotracheitis virus in specific pathogen free and broiler chickens following in ovo and subcutaneous vaccination.Gimeno, I.M., A.L. Cortes, J.S. Guy, E. Turpin, C. Williams | Avian Pathology, 40:395-403
  • (2011) Effect of diluting Marek’s disease vaccines on the outcomes of Marek’s disease virus infection when challenged with highly virulent Marek’s disease viruses.Gimeno, I.M., A.L. Cortes, E.R. Montiel, S. Lemiere, A.R. Pandiri | Avian Diseases, 55:263-272.
  • (2011). Comparison of blood and feather pulp samples for the diagnosis of Marek’s disease and for monitoring Marek’s disease vaccination by real time PCR.Cortes, A.L., E.R. Montiel, S. Lemiere, I.M. Gimeno | Avian Diseases, 55:302-310.
  • (2011) Chronological study of cytokine transcription in the spleen and lung after vaccination with serotype 1 Marek’s disease vaccines,I.M. Gimeno, A.L. Cortes | Vaccine, 29:1583-94.
  • Witter (2012) Dynamic equilibrium of Marek's disease genomes during in vitro serial passage. | Virus Genes, 45:526-36.
  • Enfermedad de Marek. 2009.Gimeno, I.M. | In Principios Basicos de Patologia Aviar. A.J.P. Ferreira and L. Revolledo (eds). Guanabara Koogan, Brasil pp. 193-228
  • 2010 Tumor diagnosis manual. The differential diagnosis of lymphoid and myeloid tumors in the chicken. | First edition, American Association of Avian Pathologists. Jacksonville, Florida.
  • (2012) Cost effective and time efficient measurement of CD4, CD8, major histocompatibility complex class II, and macrophage antigen expression in the lungs of chickens.Fletcher, O.J., X. Tan, A.L. Cortes, I.M. Gimeno | Veterinary Immunology and Immunopathology, 146: 225-236