Diagnostic Testing Lab
Department of Population Health and Pathobiology
The Histology Laboratory located in the College of Veterinary Medicine at NC State University is a full service laboratory specializing in animal tissues. The laboratory occupies 1300 square feet of Lab spaces and an additional 500 feet of archival space. We provide paraffin embedment and microtomy, frozen sectioning, special staining, immunohistochemistry and glycol methacrylate embedment processing for all species of animals. In conjunction with the Anatomic Pathology group, we provide advanced diagnostic services to the CVM teaching hospital, veterinary clinics and support residency training in anatomic pathology.
Specimen Submission Guidelines
- Containers must be labeled with names of the veterinarian, owner, and animal. If multiple samples are submitted from one animal, each tissue should be submitted in a separate container and labeled appropriately
- For margins check on mass excisions, please identify margins with sutures or a tissue ink and specify on submission form locations of each. Special stains if needed are included in the price of the original submission. If your case can benefit from ancillary immunohistochemistry testing, recommendations are in included in the report. You may call Histology, 919 513-6390 to authorize the additional testing.
- Tissues allowed to fix for 24 hour in appropriate amounts of formalin can be submitted in reduced amounts of fixative, decreasing the size and weight of the container and shipping requirements.
Surgical Pathology Samples
- Biopsy and masses with surgical margins
- Liver Pathology
- Histopathology of formalin fixed necropsy tissues are accepted. Gross necropsies are not performed on referral cases
Descriptions of Test complexities
Send In Slide Review
Second opinion on a surgical pathology case (not cytology. Up to 2 H&E slides read for one fee. Slides from outside reference laboratory should come directly to the CVM Histology Laboratory. Does not include special stains and Immunohistochemistry
Biopsies 1-2 sites
Punch, endoscopic, tru-cut biopsies or lesions less than 2cm in diameter. Up to 2 different anatomic sites. Examples include: GI biopsies from multiple areas of intestine, skin punch biopsies from 3 different sites on body, biopsies from different liver lobes, also includes feline and canine enucleation. Small excisional biopsies nodules less than 2 cm.
Additional Biopsy site
Same type as above
Mass <5 cm
any mass or organ 5 cm or less in diameter. This is a per mass charge. If it is determined that the number of cassettes are less than expected we may charge additional masses less (as an additional biopsy instead).
Mass > 5 cm
Any mass or organ greater than 5 cm in diameter. This is a per mass charge. If it is determined that the number of cassettes are less than expected we may charge additional masses less as an additional biopsy instead).
One liver biopsy with special liver panel stains
Send in necropsy tissues
Up to 8 tissues from one animal trimmed and fixed at RDVM. We do not accept fresh tissue or whole animal necropsies
Whole spleen, liver biopsy- mass> 5 cm, one additional site
Multiple small nodules ( 2cm or less) from one animal- mass < 5 cm, additional biopsy sites for others
Mammary tumors, mast cell, etc- per size of mass, if one large chain with multiple masses then charged as up to 2 masses >5 cm
Gall bladder and liver biopsy – Mass<5 cm, one additional site
More than 8 tissues in necropsy – each tissue charges as an additional biopsy site
Three lesions <2cm, labeled as 3 separate sites, (ie head, foot,back) charged as Biopsy 1-2 sites and one additional biopsy site
Click Here for Surgical Pathology Submission form
We strongly recommend you call ahead for information regarding the appropriate submission of research samples to the laboratory. Please fill out the Research Submission Form (downloadable below) and bring two (2) copies along with your histology materials.
- Research Techniques include:
- Routine paraffin processing including special stains and immunohistochemistry*Plastic (Glycol Methacrylate) embedment for soft tissues, sectioning and staining at 2 microns
- Frozen section services including snap freezing of fresh soft tissues, and infiltration and embedment of fixed and decaled specimens
- Provides training to graduate students and laboratory personnel in histologic and immunohistochemistry protocols
- Development of protocols for new antibodies
Research and Teaching Submission Guidelines electronic file
Research Account Set up Electronic file
Current Research price list Electronic file
Development of IHC protocols antibody validations – instructions electronic file