Poultry Tumor Diseases
Overview
The Poultry Tumor Diseases Laboratory (PTDL) provides tumor disease and diagnostic expertise to a variety of internal and external funded avian disease research projects, especially involving Marek’s disease. Some fee for service diagnostic work is also performed with prior approval.
Submitting Samples
Please follow the guidelines below when submitting samples.
| Tests | Description | Required Sample |
|---|---|---|
| Marek's disease diagnosis | Real time PCR | Tumor frozen (shipped in dry ice) or Tumor imprints collected in FTA cards (shipped at room temperature) |
| Monitoring Marek's disease vaccines serotype 2 | Real time PCR | Feathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature) |
| Monitoring Marek's disease vaccines serotype 3 | Real time PCR | Feathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature) |
| Monitoring Marek's disease vaccines serotype 1 | Real time PCR | Feathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature) |
| Marek's disease diagnosis and monitoring one vaccine type | Real time PCR | Tumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature) |
| Marek's disease diagnosis and monitoring of two vaccines types | Real time PCR | Tumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature) |
| Marek's disease diagnosis and monitoring of three vaccines types | Real time PCR | Tumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature) |
| Marek's disease Gene expression | Real time PCR | Contact laboratory for further specifications |
| Avian tumor histopathology | H&E reading | Tissues collected in formalin 10% |
| Marek's disease virus isolation | Plaque assay | Live animals or Frozen buffy coats (contact laboratory for instructions) |
| Marek's disease vaccine titration (monovalent) | Plaque assay | Three vaccine vials in liquid shipped in nitrogen or dry ice |
| Marek's disease vaccine titration (bivalent) | Plaque assay | Three vaccine vials in liquid shipped in nitrogen or dry ice |
| Marek's disease vaccine titration(trivalent) | Plaque assay | Three vaccine vials in liquid shipped in nitrogen or dry ice |
| Differential diagnosis avian tumors | Real time PCR: Marek's disease Virus isolation: REV, ALV PCR: REV, ALV | Tumors frozen (shipped in dry ice) and frozen plasma |
| Immunohistochemistry (MD, cell phenotype) | Immunohistochemistry | Tissues frozen in OCT and shipped in dry ice (contact laboratory for instructions) |
| Pathotyping | Gold standard pathotyping assay | Live animals or Frozen buffy coats (contact laboratory for instructions) or Marek’s disease virus in cell culture or Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice |
| Neuropathotyping | Neuro signs | Live animals or Frozen buffy coats (contact laboratory for instructions) or Marek’s disease virus in cell culture or Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice |
| Peripheral neuropathy diagnosis | Real time PCR Histopathology | Frozen nerves shipped in dry ice and nerves fixed in formalin 10% |
Required Paperwork
Sample Submission Form – Poultry Tumor Diseases Laboratory
Click to download form – fee schedule is included – to complete and submit with samples.
Ship samples to:
Attn. Lab Supervisor
North Carolina State University
College of Veterinary Medicine
1060 William Moore Drive
Raleigh, NC 27607 USA
