Diagnostic Testing and Research Lab
Department of Population Health and Pathobiology
Poultry Tumor Diseases
Lab Contact: 919.513.6333
The Poultry Tumor Diseases Laboratory (PTDL) provides tumor disease and diagnostic expertise to a variety of internal and external funded avian disease research projects, especially involving Marek’s disease. Some fee for service diagnostic work is also performed with prior approval.
Submitting Samples
Please follow the guidelines below when submitting samples.
Tests
Description
Required Sample
Marek’s disease diagnosis
Real time PCR
Tumor frozen (shipped in dry ice) or Tumor imprints collected in FTA cards (shipped at room temperature)
Monitoring Marek’s disease vaccines serotype 2
Real time PCR
Feathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature)
Monitoring Marek’s disease vaccines serotype 3
Real time PCR
Feathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature)
Monitoring Marek’s disease vaccines serotype 1
Real time PCR
Feathers frozen (shipped in dry ice) or Feather pulp collected in FTA cards (shipped at room temperature)
Marek’s disease diagnosis and monitoring one vaccine type
Real time PCR
Tumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature)
Marek’s disease diagnosis and monitoring of two vaccines types
Real time PCR
Tumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature)
Marek’s disease diagnosis and monitoring of three vaccines types
Real time PCR
Tumors and feathers frozen (shipped in dry ice) or tumor imprint and feather pulp collected in FTA cards (shipped at room temperature)
Marek’s disease Gene expression
Real time PCR
Contact laboratory for further specifications
Avian tumor histopathology
H&E reading
Tissues collected in formalin 10%
Marek’s disease virus isolation
Plaque assay
Live animals or Frozen buffy coats (contact laboratory for instructions)
Marek’s disease vaccine titration (monovalent)
Plaque assay
Three vaccine vials in liquid shipped in nitrogen or dry ice
Marek’s disease vaccine titration (bivalent)
Plaque assay
Three vaccine vials in liquid shipped in nitrogen or dry ice
Marek’s disease vaccine titration(trivalent)
Plaque assay
Three vaccine vials in liquid shipped in nitrogen or dry ice
Differential diagnosis avian tumors
Real time PCR: Marek’s disease
Virus isolation: REV, ALV PCR: REV, ALV
Virus isolation: REV, ALV PCR: REV, ALV
Tumors frozen (shipped in dry ice) and frozen plasma
Immunohistochemistry (MD, cell phenotype)
Immunohistochemistry
Tissues frozen in OCT and shipped in dry ice (contact laboratory for instructions)
Pathotyping
Gold standard pathotyping assay
Live animals
or
Frozen buffy coats (contact laboratory for instructions)
or
Marek’s disease virus in cell culture
or
Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice
or
Frozen buffy coats (contact laboratory for instructions)
or
Marek’s disease virus in cell culture
or
Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice
Neuropathotyping
Neuro signs
Live animals
or
Frozen buffy coats (contact laboratory for instructions)
or
Marek’s disease virus in cell culture
or
Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice
or
Frozen buffy coats (contact laboratory for instructions)
or
Marek’s disease virus in cell culture
or
Marek’s disease virus frozen in liquid nitrogen and shipped in dry ice
Peripheral neuropathy diagnosis
Real time PCR Histopathology
Frozen nerves shipped in dry ice and nerves fixed in formalin 10%
Required Paperwork
Click to download form – fee schedule is included – to complete and submit with samples.
Ship samples to:
Attn. Lab Supervisor
North Carolina State University
College of Veterinary Medicine
1060 William Moore Drive
Raleigh, NC 27607 USA
North Carolina State University
College of Veterinary Medicine
1060 William Moore Drive
Raleigh, NC 27607 USA
